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Literature DB >> 17898161

Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection.

Christelle Vauloup-Fellous¹, Aurélie Ducroux, Vincent Couloigner, Sandrine Marlin, Olivier Picone, Julie Galimand, Natalie Loundon, Françoise Denoyelle, Liliane Grangeot-Keros, Marianne Leruez-Ville.

Abstract

We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.

Entities: Disease

Mesh：

Substances：
DNA, Viral

Year: 2007 PMID： 17898161 PMCID： PMC2168533 DOI： 10.1128/JCM.01654-07

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

17 in total

1. Cytomegalovirus DNA detection in Guthrie cards: a powerful tool for diagnosing congenital infection.

Authors: M Barbi; S Binda; V Primache; S Caroppo; P Didò; P Guidotti; C Corbetta; D Melotti
Journal: J Clin Virol Date: 2000-09-01 Impact factor: 3.168

2. Retrospective diagnostics of congenital cytomegalovirus infection performed by polymerase chain reaction in blood stored on filter paper.

Authors: P J Johansson; M Jönsson; K Ahlfors; S A Ivarsson; L Svanberg; C Guthenberg
Journal: Scand J Infect Dis Date: 1997

3. Should we routinely screen for cytomegalovirus antibody during pregnancy?

Authors: L Grangeot-Keros; B Simon; F Audibert; M Vial
Journal: Intervirology Date: 1998 Impact factor: 1.763

4. [Optimisation of retrospective diagnosis of cytomegalovirus congenital infection from dried blood spots].

Authors: C Vauloup-Fellous; P Dubreuil; L Grangeot-Keros
Journal: Pathol Biol (Paris) Date: 2006-10-05

5. Abnormal white matter lesions with sensorineural hearing loss caused by congenital cytomegalovirus infection: retrospective diagnosis by PCR using Guthrie cards.

Authors: Kazuhiro Haginoya; Toshihiro Ohura; Kimiya Kon; Tsuneo Yagi; Yukio Sawaishi; Keiko Kumura Ishii; Tadao Funato; Shuichi Higano; Shoki Takahashi; Kazuie Iinuma
Journal: Brain Dev Date: 2002-10 Impact factor: 1.961

6. Usefulness of blood and urine samples collected on filter paper in detecting cytomegalovirus by the polymerase chain reaction technique.

Authors: A Y Yamamoto; M M Mussi-Pinhata; P C Pinto; L T Figueiredo; S M Jorge
Journal: J Virol Methods Date: 2001-09 Impact factor: 2.014

7. Modification of CMV DNA detection from dried blood spots for diagnosing congenital CMV infection.

Authors: Sandro Binda; Simona Caroppo; Patrizia Didò; Valeria Primache; Licia Veronesi; Agata Calvario; Andrea Piana; Maria Barbi
Journal: J Clin Virol Date: 2004-07 Impact factor: 3.168

8. Retrospective diagnosis of congenital cytomegalovirus infection and cortical maldevelopment.

Authors: C Zucca; S Binda; R Borgatti; F Triulzi; L Radice; C Buttè; P E Barkhaus; M Barbi
Journal: Neurology Date: 2003-09-09 Impact factor: 9.910

Review 9. Diagnosis and management of human cytomegalovirus infection in the mother, fetus, and newborn infant.

Authors: Maria Grazia Revello; Giuseppe Gerna
Journal: Clin Microbiol Rev Date: 2002-10 Impact factor: 26.132

10. Human cytomegalovirus: detection of congenital and perinatal infection in Argentina.

Authors: Angélica Lidia Distéfano; Alicia Alonso; Fabián Martin; Fabián Pardon
Journal: BMC Pediatr Date: 2004-06-23 Impact factor: 2.125

12 in total

Review 1. Public health and laboratory considerations regarding newborn screening for congenital cytomegalovirus.

Authors: Sheila C Dollard; Mark R Schleiss; Scott D Grosse
Journal: J Inherit Metab Dis Date: 2010-06-08 Impact factor: 4.982

2. Rapid genotyping of cytomegalovirus in dried blood spots by multiplex real-time PCR assays targeting the envelope glycoprotein gB and gH genes.

Authors: Jutte J C de Vries; Els Wessels; Anna M H Korver; Annemiek A van der Eijk; Lisette G Rusman; Aloys C M Kroes; Ann C T M Vossen
Journal: J Clin Microbiol Date: 2011-11-23 Impact factor: 5.948

Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection.

1. Cytomegalovirus DNA detection in Guthrie cards: a powerful tool for diagnosing congenital infection.

2. Retrospective diagnostics of congenital cytomegalovirus infection performed by polymerase chain reaction in blood stored on filter paper.

3. Should we routinely screen for cytomegalovirus antibody during pregnancy?

4. [Optimisation of retrospective diagnosis of cytomegalovirus congenital infection from dried blood spots].

5. Abnormal white matter lesions with sensorineural hearing loss caused by congenital cytomegalovirus infection: retrospective diagnosis by PCR using Guthrie cards.

6. Usefulness of blood and urine samples collected on filter paper in detecting cytomegalovirus by the polymerase chain reaction technique.

7. Modification of CMV DNA detection from dried blood spots for diagnosing congenital CMV infection.

8. Retrospective diagnosis of congenital cytomegalovirus infection and cortical maldevelopment.

Review 9. Diagnosis and management of human cytomegalovirus infection in the mother, fetus, and newborn infant.

10. Human cytomegalovirus: detection of congenital and perinatal infection in Argentina.

Review 1. Public health and laboratory considerations regarding newborn screening for congenital cytomegalovirus.

2. Rapid genotyping of cytomegalovirus in dried blood spots by multiplex real-time PCR assays targeting the envelope glycoprotein gB and gH genes.

Review 3. The "silent" global burden of congenital cytomegalovirus.

4. Evaluation of DNA extraction methods for the detection of Cytomegalovirus in dried blood spots.

5. Incidence of CMV co-infection in HIV-positive women and their neonates in a tertiary referral centre: a cohort study.

6. Prevention of maternal cytomegalovirus infection: current status and future prospects.

7. Quantitation of cytomegalovirus DNA load in dried blood spots correlates well with plasma viral load.

Review 8. Congenital and perinatal infections: throwing new light with an old TORCH.

9. Prevalence of congenital cytomegalovirus infection in symptomatic newborns under 3 weeks in Tehran, Iran.

Review 10. Cytomegalovirus in pregnancy and the neonate.