| Literature DB >> 17898064 |
Yongsheng Jin1, Dongyuan Ma, Jiangli Dong, Jingchen Jin, Daofeng Li, Changwang Deng, Tao Wang.
Abstract
The multifunctional protein helper component proteinase (HC-Pro) is thought to interfere with the activity of the 20S proteasome; however, no sites of interaction have been identified for either protein. Here, we first show that the Potato virus Y (PVY) HC-Pro protein can interact with three Arabidopsis 20S proteasome subunits (PAA, PBB, and PBE), using a yeast two-hybrid system and the bimolecular fluorescence complement assay. In addition, yeast two-hybrid analysis of the interaction between several mutant subunits of the 20S proteasome and PVY HC-Pro confirmed that residues 81 to 140 of PAA, 1 to 80 of PBB, and 160 to 274 of PBE are necessary for binding PAA, PBB, and PBE to PVY HC-Pro, respectively. Deletion mutant analysis of PVY HC-Pro showed that the N terminus (residues 1 to 97) is necessary for its interaction with three Arabidopsis 20S proteasome subunits. The ability of HC-Pro to interact and interfere with the activity of the 20S proteasome may help explain the molecular basis of its multifunctional character.Entities:
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Year: 2007 PMID： 17898064 PMCID： PMC2169110 DOI： 10.1128/JVI.00913-07
Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103