OBJECTIVES: Polymerized resin composites and nonpolymerized monomers are reported to accelerate bacterial growth. Furthermore, in vivo, resin composite restorations accumulate more plaque than other restorative materials. The purpose of this study was to test the hypothesis that bacteria-composite surface interaction causes changes in surface-topography. METHODS: Resin composite disks were polymerized between two glass slides. Streptococcus mutans cells were brought in contact with and grown on the disks for 1 day, 1 week or 1 month. The disks were analyzed using atomic force microscopy. One-month-aged composite specimens were assayed for changes in micro-hardness and bacterial outgrowth. RESULTS: Atomic force microscopy analysis revealed a time-dependent increase in root mean square (RMS) roughness (p<0.0001). S. mutans outgrowth was accelerated following direct contact with the surface of aged composites, with no changes in micro-hardness. SIGNIFICANCE: Our results show that S. mutans growth on resin composite increases surface roughness without affecting micro-hardness. The change in surface integrity may further accelerate biofilm accumulation.
OBJECTIVES: Polymerized resin composites and nonpolymerized monomers are reported to accelerate bacterial growth. Furthermore, in vivo, resin composite restorations accumulate more plaque than other restorative materials. The purpose of this study was to test the hypothesis that bacteria-composite surface interaction causes changes in surface-topography. METHODS: Resin composite disks were polymerized between two glass slides. Streptococcus mutans cells were brought in contact with and grown on the disks for 1 day, 1 week or 1 month. The disks were analyzed using atomic force microscopy. One-month-aged composite specimens were assayed for changes in micro-hardness and bacterial outgrowth. RESULTS: Atomic force microscopy analysis revealed a time-dependent increase in root mean square (RMS) roughness (p<0.0001). S. mutans outgrowth was accelerated following direct contact with the surface of aged composites, with no changes in micro-hardness. SIGNIFICANCE: Our results show that S. mutans growth on resin composite increases surface roughness without affecting micro-hardness. The change in surface integrity may further accelerate biofilm accumulation.
Authors: Nurit Beyth; Ira Yudovin-Farber; Michael Perez-Davidi; Abraham J Domb; Ervin I Weiss Journal: Proc Natl Acad Sci U S A Date: 2010-12-03 Impact factor: 11.205
Authors: Hamad Algamaiah; Robert Danso; Jeffrey Banas; Steve R Armstrong; Kyumin Whang; H Ralph Rawls; Erica C Teixeira Journal: Clin Oral Investig Date: 2019-05-18 Impact factor: 3.573
Authors: Mustafa Murat Mutluay; Ke Zhang; Heonjune Ryou; Mobin Yahyazadehfar; Hessam Majd; Hockin H K Xu; Dwayne Arola Journal: J Mech Behav Biomed Mater Date: 2012-11-17