Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Low- to high-throughput analysis of telomerase modulators with Telospot.

Literature DB >> 17893679

Low- to high-throughput analysis of telomerase modulators with Telospot.

Gaël Cristofari¹, Patrick Reichenbach, Pierre-Olivier Regamey, Damiano Banfi, Marc Chambon, Gerardo Turcatti, Joachim Lingner.

Abstract

We designed a method termed Telospot to discover and characterize telomerase modulators as anticancer drugs or chemical biology tools. Telospot is based on a highly efficient human telomerase expression system and the detection of telomerase DNA reaction products in macroarray format. Telospot offers a highly scalable, cost- and time-effective alternative to presently available telomerase assays, which are limited by the requirement for PCR, telomerase purification or technologies not amenable to high throughput.

Entities: Species

Mesh：

Substances：

Year: 2007 PMID： 17893679 DOI： 10.1038/nmeth1099

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

Keyword Cloud
Cited

12 in total

1. Structural Analysis Reveals the Deleterious Effects of Telomerase Mutations in Bone Marrow Failure Syndromes.

Authors: Hunter Hoffman; Cory Rice; Emmanuel Skordalakes
Journal: J Biol Chem Date: 2017-02-01 Impact factor: 5.157

2. The non-coding RNA TERRA is a natural ligand and direct inhibitor of human telomerase.

Authors: Sophie Redon; Patrick Reichenbach; Joachim Lingner
Journal: Nucleic Acids Res Date: 2010-05-11 Impact factor: 16.971

3. POT1-TPP1 enhances telomerase processivity by slowing primer dissociation and aiding translocation.

Authors: Chrysa M Latrick; Thomas R Cech
Journal: EMBO J Date: 2010-01-21 Impact factor: 14.012

4. The human CST complex is a terminator of telomerase activity.

Authors: Liuh-Yow Chen; Sophie Redon; Joachim Lingner
Journal: Nature Date: 2012-08-23 Impact factor: 69.504

Review 5. Targeting the telomere and shelterin complex for cancer therapy: current views and future perspectives.

Authors: Alan E Bilsland; Claire J Cairney; W Nicol Keith
Journal: J Cell Mol Med Date: 2011-02 Impact factor: 5.310

6. Identification of G-Quadruplex Inducers Usinga Simple, Inexpensiveand Rapid High Throughput Assay, and TheirInhibition of Human Telomerase.

Authors: Maria Florencia Sassano; Alexander P Schlesinger; Michael B Jarstfer
Journal: Open Med Chem J Date: 2012-10-19

Low- to high-throughput analysis of telomerase modulators with Telospot.

1. Structural Analysis Reveals the Deleterious Effects of Telomerase Mutations in Bone Marrow Failure Syndromes.

2. The non-coding RNA TERRA is a natural ligand and direct inhibitor of human telomerase.

3. POT1-TPP1 enhances telomerase processivity by slowing primer dissociation and aiding translocation.

4. The human CST complex is a terminator of telomerase activity.

Review 5. Targeting the telomere and shelterin complex for cancer therapy: current views and future perspectives.

6. Identification of G-Quadruplex Inducers Usinga Simple, Inexpensiveand Rapid High Throughput Assay, and TheirInhibition of Human Telomerase.

7. A three-state model for the regulation of telomerase by TERRA and hnRNPA1.

8. Structure of active dimeric human telomerase.

Review 9. Telomere maintenance mechanisms in cancer: clinical implications.

10. Structural and functional analysis of the human POT1-TPP1 telomeric complex.