G M Kikuvi1, J N Ombui, E S Mitema, S Schwarz. 1. Institute of Tropical Medicine and Infectious Diseases (ITROMID), Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.
Abstract
OBJECTIVES: To isolate Salmonella from food animals and characterise the antimicrobial resistance of the isolates. DESIGN: A random sampling of slaughter animals was carried out. SETTING: Department of Public Health, Pharmacology and Toxicology, University of Nairobi, Kenya and Institute for Animal Breeding, Neustadt-Mariensee, Germany. SUBJECTS: Two hundred and eighty five samples, including faecal samples and carcass, cloacal and pharyngeal swab samples were analysed. RESULTS: Sixteen (5.6%) of 285 samples were positive for Salmonella. The prevalence of Salmonella on pig carcasses (19%) was higher than in faeces (8.6%). Three Salmonella enterica sub-species enterica serovars, namely Saintpaul (S. Saintpaul), Braenderup (S. Braenderup), and Heidelberg (S. Heidelberg), were identified, with S. Saintpaul being the predominant serovar. Antimicrobial resistance was found in 35.7% of all the isolates. The S. Heidelberg isolates were susceptible to all the antimicrobial agents tested. Multidrug resistance was found in 7.1% of the resistant Salmonella isolates. Plasmids were only detected in S. Heidelberg. Ampicillin resistance was based on expression of a bla(TEM) gene, while chloramphenicol, streptomycin, and tetracycline resistances were encoded by the genes catAl, strA, and tet(A), respectively. CONCLUSION: Pigs may serve as reservoirs of antimicrobial resistant Salmonella and slaughterhouse cross-contamination of pork may be a food safety risk. We recommended that slaughterhouse hygiene be improved to minimise contamination of pig carcasses.
OBJECTIVES: To isolate Salmonella from food animals and characterise the antimicrobial resistance of the isolates. DESIGN: A random sampling of slaughter animals was carried out. SETTING: Department of Public Health, Pharmacology and Toxicology, University of Nairobi, Kenya and Institute for Animal Breeding, Neustadt-Mariensee, Germany. SUBJECTS: Two hundred and eighty five samples, including faecal samples and carcass, cloacal and pharyngeal swab samples were analysed. RESULTS: Sixteen (5.6%) of 285 samples were positive for Salmonella. The prevalence of Salmonella on pig carcasses (19%) was higher than in faeces (8.6%). Three Salmonella enterica sub-species enterica serovars, namely Saintpaul (S. Saintpaul), Braenderup (S. Braenderup), and Heidelberg (S. Heidelberg), were identified, with S. Saintpaul being the predominant serovar. Antimicrobial resistance was found in 35.7% of all the isolates. The S. Heidelberg isolates were susceptible to all the antimicrobial agents tested. Multidrug resistance was found in 7.1% of the resistant Salmonella isolates. Plasmids were only detected in S. Heidelberg. Ampicillin resistance was based on expression of a bla(TEM) gene, while chloramphenicol, streptomycin, and tetracycline resistances were encoded by the genes catAl, strA, and tet(A), respectively. CONCLUSION:Pigs may serve as reservoirs of antimicrobial resistant Salmonella and slaughterhouse cross-contamination of pork may be a food safety risk. We recommended that slaughterhouse hygiene be improved to minimise contamination of pig carcasses.
Authors: Alaa Eldin M A Morshdy; Ahmed S El-Tahlawy; Sameer H Qari; Alaa T Qumsani; Daniyah Habiballah Bay; Rokayya Sami; Eman Hillal Althubaiti; Ahmed M A Mansour; Amani H Aljahani; Abd El-Salam E Hafez; Abdallah Fikry A Mahmoud; Rasha M El Bayomi; Mohamed A Hussein Journal: Molecules Date: 2022-03-28 Impact factor: 4.411
Authors: Kate M Thomas; William A de Glanville; Gary C Barker; Jackie Benschop; Joram J Buza; Sarah Cleaveland; Margaret A Davis; Nigel P French; Blandina T Mmbaga; Gerard Prinsen; Emmanuel S Swai; Ruth N Zadoks; John A Crump Journal: Int J Food Microbiol Date: 2019-10-31 Impact factor: 5.277