A Papathanasiou1, O Djahanbakhch, E Saridogan, R A Lyons. 1. Academic Department of Obstetrics and Gynaecology, Bart's and The London and Newham University Hospitals and Queen Mary's School of Medicine and Dentistry, London, United Kingdom.
Abstract
OBJECTIVE: To investigate the effect of interleukin-6 (IL-6) on ciliary activity within the human fallopian tube in vitro. DESIGN: An experimental laboratory-based study. SETTING: University teaching hospital. PATIENT(S): Fallopian tube specimens were obtained from eight women undergoing hysterectomy for fibroid uterus. MAIN OUTCOME MEASURE(S): Ciliary beat frequency (CBF) in the human fallopian tube. INTERVENTION(S): After baseline CBF measurements, increasing concentrations of IL-6 (10 pg/mL, 100 pg/mL, and 1000 pg/mL) were applied to fallopian tube mucosa explants and CBF measurements repeated. An anti-IL-6 monoclonal antibody was added and CBF measured once more. Negative and antibody-only control samples were used. RESULT(S): The CBF remained unchanged with the addition of IL-6 at concentrations of 10 pg/mL and 100 pg/mL but IL-6 at 1000 pg/mL significantly decreased CBF, an effect abolished by the addition of the specific antibody. CONCLUSION(S): These data suggest that IL-6 has an inhibitory effect on ciliary activity, suggesting a possible role for this cytokine in the subfertility process. This finding may be important in conditions where there are increased IL-6 levels in the peritoneal fluid, such as endometriosis and pelvic inflammatory disease.
OBJECTIVE: To investigate the effect of interleukin-6 (IL-6) on ciliary activity within the human fallopian tube in vitro. DESIGN: An experimental laboratory-based study. SETTING: University teaching hospital. PATIENT(S): Fallopian tube specimens were obtained from eight women undergoing hysterectomy for fibroid uterus. MAIN OUTCOME MEASURE(S): Ciliary beat frequency (CBF) in the human fallopian tube. INTERVENTION(S): After baseline CBF measurements, increasing concentrations of IL-6 (10 pg/mL, 100 pg/mL, and 1000 pg/mL) were applied to fallopian tube mucosa explants and CBF measurements repeated. An anti-IL-6 monoclonal antibody was added and CBF measured once more. Negative and antibody-only control samples were used. RESULT(S): The CBF remained unchanged with the addition of IL-6 at concentrations of 10 pg/mL and 100 pg/mL but IL-6 at 1000 pg/mL significantly decreased CBF, an effect abolished by the addition of the specific antibody. CONCLUSION(S): These data suggest that IL-6 has an inhibitory effect on ciliary activity, suggesting a possible role for this cytokine in the subfertility process. This finding may be important in conditions where there are increased IL-6 levels in the peritoneal fluid, such as endometriosis and pelvic inflammatory disease.
Authors: Xia Wang; Cheuk-Lun Lee; Madhavi Vijayan; William S B Yeung; Ernest H Y Ng; Xiwen Wang; Wai-Sum O; Raymond H W Li; Yuanzhen Zhang; Philip C N Chiu Journal: Mucosal Immunol Date: 2020-03-13 Impact factor: 7.313
Authors: Moira L Aitken; Ranjani Somayaji; Thomas R Hinds; Maricela Pier; Karla Droguett; Mariana Rios; Shawn J Skerrett; Manuel Villalon Journal: Front Physiol Date: 2021-04-23 Impact factor: 4.566