| Literature DB >> 17885925 |
Yoshifumi Fujita1, Tomoko Takahashi, Akiko Suzuki, Kayo Kawashima, Futoshi Nara, Ryuta Koishi.
Abstract
Dresden G protein-coupled receptor (D-GPCR) is one of orphan G protein-coupled receptors (GPCR). Here we report the identification of the ligands and the characterization of D-GPCR. We investigated over 5000 compounds to evoke the response mediated by D-GPCR and identified 3-methyl-valeric acid and 4-methyl-valeric acid as agonists using a cAMP assay. It is of interest that they dramatically enhanced the intracellular cAMP accumulation and the CRE-luciferase activity in CHO-K1 cells and HEK293 cells expressing the chimeric protein of D-GPCR with a rhodopsin-tag at its N-terminus. Our results established new characteristics of D-GPCR as an olfactory receptor. First, agonists of D-GPCR belong to odorants. Second, D-GPCR mRNA is expressed in the olfactory bulb. In addition, D-GPCR was reported to have similar sequences and its genome locus nearby other olfactory receptors. These results suggest D-GPCR is an olfactory receptor.Entities:
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Year: 2007 PMID： 17885925 DOI： 10.1080/10799890701534180
Source DB: PubMed Journal: J Recept Signal Transduct Res ISSN： 1079-9893 Impact factor: 2.092