Jun-Sheng Li1, Guang-Wen Sun, Xiao-Ying Wei, Wen-Hao Tang. 1. Department of General Surgery, Affiliated Zhong-Da Hospital, Southeast University, Nanjing 210009, Jiangsu Province, China. lijunshenghd@126.com
Abstract
AIM: To investigate the expression and localization of periostin in gastric cancer and its clinical relevance. METHODS: Reverse transcriptase polymerase chain reaction was used to measure periostin mRNA expression. Western blotting was carried out to detect periostin protein expression. Immunohistochemistry was performed to localize and quantify the expression of periostin in benign gastric diseases and gastric cancer, and immunostaining results were correlated with gastric cancer pathological stages. RESULTS: Periostin expression was low at both mRNA and protein levels in normal gastric tissues, but was overexpressed in gastric cancer tissues. Immunohistochemical staining revealed that periostin was overexpressed in primary gastric cancer, as well as in metastatic lymph nodes, but only faint staining was found in benign gastric ulcers. By quantitative analysis of the immunostaining results, periostin expression was increased 2.5-4-fold in gastric cancer, compared to that in benign gastric disease, and there was a trend toward increasing periostin expression with tumor stage. CONCLUSION: This observation demonstrated that periostin was overexpressed in gastric cancer and lymph node metastasis, which suggests that periostin plays an important role in the progression and metastasis of gastric cancer.
AIM: To investigate the expression and localization of periostin in gastric cancer and its clinical relevance. METHODS: Reverse transcriptase polymerase chain reaction was used to measure periostin mRNA expression. Western blotting was carried out to detect periostin protein expression. Immunohistochemistry was performed to localize and quantify the expression of periostin in benign gastric diseases and gastric cancer, and immunostaining results were correlated with gastric cancer pathological stages. RESULTS:Periostin expression was low at both mRNA and protein levels in normal gastric tissues, but was overexpressed in gastric cancer tissues. Immunohistochemical staining revealed that periostin was overexpressed in primary gastric cancer, as well as in metastatic lymph nodes, but only faint staining was found in benign gastric ulcers. By quantitative analysis of the immunostaining results, periostin expression was increased 2.5-4-fold in gastric cancer, compared to that in benign gastric disease, and there was a trend toward increasing periostin expression with tumor stage. CONCLUSION: This observation demonstrated that periostin was overexpressed in gastric cancer and lymph node metastasis, which suggests that periostin plays an important role in the progression and metastasis of gastric cancer.
Authors: H Sasaki; M Dai; D Auclair; M Kaji; I Fukai; M Kiriyama; Y Yamakawa; Y Fujii; L B Chen Journal: Cancer Lett Date: 2001-10-22 Impact factor: 8.679
Authors: K Horiuchi; N Amizuka; S Takeshita; H Takamatsu; M Katsuura; H Ozawa; Y Toyama; L F Bonewald; A Kudo Journal: J Bone Miner Res Date: 1999-07 Impact factor: 6.741
Authors: Volkhard Lindner; Qiaozeng Wang; Barbara A Conley; Robert E Friesel; Calvin P H Vary Journal: Arterioscler Thromb Vasc Biol Date: 2004-10-28 Impact factor: 8.311
Authors: Hidefumi Sasaki; Chih-Yi Yu; Meiru Dai; Carmen Tam; Massimo Loda; Daniel Auclair; Lan Bo Chen; Anthony Elias Journal: Breast Cancer Res Treat Date: 2003-02 Impact factor: 4.872
Authors: Lindsay Gillan; Daniela Matei; David A Fishman; C S Gerbin; Beth Y Karlan; David D Chang Journal: Cancer Res Date: 2002-09-15 Impact factor: 12.701
Authors: Shideng Bao; Gaoliang Ouyang; Xuefang Bai; Zhi Huang; Chaoyu Ma; Ming Liu; Rong Shao; Ryan M Anderson; Jeremy N Rich; Xiao-Fan Wang Journal: Cancer Cell Date: 2004-04 Impact factor: 31.743
Authors: Laura Morra; Markus Rechsteiner; Silvia Casagrande; Van Duc Luu; Roger Santimaria; Pierre A Diener; Tullio Sulser; Glen Kristiansen; Peter Schraml; Holger Moch; Alex Soltermann Journal: Am J Pathol Date: 2011-07-18 Impact factor: 4.307
Authors: Tadeusz Moniuszko; Andrzej Wincewicz; Mariusz Koda; Izabela Domysławska; Stanisław Sulkowski Journal: Oncol Lett Date: 2016-06-09 Impact factor: 2.967