BACKGROUND AND OBJECTIVES: Endothelin-1 (ET-1), a potent vasoconstrictor peptide produced by endothelial cells, has been implicated in the dysfunction of various organs. To determine the role of ET-1 in acute lung injury (ALI) and ARDS, ET-1 levels were measured in epithelial lining fluid (ELF) and plasma obtained from patients with ALI/ARDS. METHODS: A cross-sectional study of patients with ALI/ARDS in the intensive care unit of two university hospitals was performed. Patients with ALI/ARDS underwent bronchoscopic microsampling to collect ELF on the day of onset of the disease. Patients who underwent bronchoscopy to examine a small peripheral pulmonary nodule served as controls. RESULTS: In the 23 patients with ALI/ARDS, the ET-1 level in ELF was significantly greater than that in plasma (P < 0.001). In contrast, ET-1 was not detectable in the ELF from six of the seven control subjects. The albumin concentration of ELF, used as a marker of endothelial and epithelial permeability, correlated with the ET-1 level in ELF (P < 0.001). The oxygenation index (PaO(2)/FiO(2)) was also correlated with ET-1 concentration in ELF (P < 0.001). CONCLUSION: In patients with ALI/ARDS, ET-1 is produced mainly in the lung and is associated not only with pulmonary vasoconstriction but also the development of permeability oedema, leading to the impairment of oxygenation.
BACKGROUND AND OBJECTIVES:Endothelin-1 (ET-1), a potent vasoconstrictor peptide produced by endothelial cells, has been implicated in the dysfunction of various organs. To determine the role of ET-1 in acute lung injury (ALI) and ARDS, ET-1 levels were measured in epithelial lining fluid (ELF) and plasma obtained from patients with ALI/ARDS. METHODS: A cross-sectional study of patients with ALI/ARDS in the intensive care unit of two university hospitals was performed. Patients with ALI/ARDS underwent bronchoscopic microsampling to collect ELF on the day of onset of the disease. Patients who underwent bronchoscopy to examine a small peripheral pulmonary nodule served as controls. RESULTS: In the 23 patients with ALI/ARDS, the ET-1 level in ELF was significantly greater than that in plasma (P < 0.001). In contrast, ET-1 was not detectable in the ELF from six of the seven control subjects. The albumin concentration of ELF, used as a marker of endothelial and epithelial permeability, correlated with the ET-1 level in ELF (P < 0.001). The oxygenation index (PaO(2)/FiO(2)) was also correlated with ET-1 concentration in ELF (P < 0.001). CONCLUSION: In patients with ALI/ARDS, ET-1 is produced mainly in the lung and is associated not only with pulmonary vasoconstriction but also the development of permeability oedema, leading to the impairment of oxygenation.
Authors: Helene M Wolsk; Bo L Chawes; Jonathan Thorsen; Jakob Stokholm; Klaus Bønnelykke; Susanne Brix; Hans Bisgaard Journal: J Vis Exp Date: 2017-08-07 Impact factor: 1.355
Authors: Priya Kulasekaran; Casey A Scavone; David S Rogers; Douglas A Arenberg; Victor J Thannickal; Jeffrey C Horowitz Journal: Am J Respir Cell Mol Biol Date: 2009-02-02 Impact factor: 6.914
Authors: Alejandro P Comellas; Arturo Briva; Laura A Dada; Maria L Butti; Humberto E Trejo; Cecilia Yshii; Zaher S Azzam; Juan Litvan; Jiwang Chen; Emilia Lecuona; Liuska M Pesce; Masashi Yanagisawa; Jacob I Sznajder Journal: Am J Respir Crit Care Med Date: 2008-10-23 Impact factor: 21.405