Literature DB >> 17870177

Laboratory production of 100 base pair DNA molecular weight markers.

Microsugar Chang1, Jhih-Hao Wang, Han-Jung Lee.   

Abstract

DNA molecular weight markers are routinely used in agarose gel electrophoresis. Here we report a method called PCR-synthesized marker (PSM) to generate DNA molecular rulers by PCR in the laboratory. This strategy can also be used to produce 100 bp RNA molecular weight markers by run-off transcription.

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Year:  2007        PMID: 17870177     DOI: 10.1016/j.jbbm.2007.08.002

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  5 in total

1.  Straightforward procedure for laboratory production of DNA ladder.

Authors:  Vo Thi Thuong Lan; Pham Thi Thanh Loan; Pham Anh Thuy Duong; Le Thi Thanh; Ngo Thi Ha; Ta Bich Thuan
Journal:  J Nucleic Acids       Date:  2012-02-12

2.  A novel combined method for cost-benefit production of DNA ladders.

Authors:  Saied Mostaan; Mehdi Ajorloo; Hossein Khanahmad; Reza Ahangari Cohan; Zahra Rikhtegaran Tehrani; Maryam Rezaei; Fateme Fazeli; Mehdi Behdani; Sakineh Karimi Zare; Zeinab Karimi; Seyed Hamid Reza Mozhgani; Rasul Moukhah
Journal:  Adv Biomed Res       Date:  2015-01-06

3.  Easy method for production of a home-made DNA ladder in every laboratory.

Authors:  Mahdi Abbasian; Hadieh Alsadat Eslampanah Seyedi; Zahra Khalili Boroujeni; Mohammad Reza Mofid
Journal:  Adv Biomed Res       Date:  2015-03-25

4.  The pPSU Plasmids for Generating DNA Molecular Weight Markers.

Authors:  Ryan C Henrici; Turner J Pecen; James L Johnston; Song Tan
Journal:  Sci Rep       Date:  2017-05-26       Impact factor: 4.379

5.  "In-house" production of DNA size marker from a vaccinal Bacillus anthracis strain.

Authors:  Mohammad Sekhavati; Keyvan Tadayon; Rainak Ghaderi; Reza Banihashemi; Ahmad Reza Jabbari; Gholamreza Shokri; Nasim Karimnasab
Journal:  Iran J Microbiol       Date:  2015-02
  5 in total

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