BACKGROUND: Osteopontin (OPN) promotes the migration and adhesion of vascular smooth muscle cells (VSMCs) through cell surface receptor, integrin beta3. In order to elucidate the signaling pathway by which OPN is involved in neointimal formation, we focused on integrin beta3-focal adhesion kinase (FAK) upon VSMC migration. METHODS: The integrin beta3 and FAK expression in VSMC and in neointima was detected by Western blot and immunohistochemistry staining. FAK phosphorylation induced by OPN was verified using a linear OPN 13 peptide containing RGD motif and anti-OPN antibody. The role of integrin beta3-FAK pathway in VSMC adhesion and migration induced with OPN was tested by the overexpression of FAK-related nonkinase and integrin beta3 cytoplasmic domain. RESULTS: The results showed that OPN increased integrin beta3 expression and induced rapid and transient FAK phosphorylation. Inhibition of the phosphorylation of FAK significantly suppressed VSMC migration induced by OPN. Similarly, blockade of the interaction of integrin beta3 with OPN inhibited VSMC adhesion induced by OPN. The experiment, in vivo, demonstrated that OPN expression level was consistent with neointimal thickening. Administration of anti-OPN antibody for blocking OPN function suppressed integrin beta3 and FAK expression induced by balloon injury, and neointimal thickening was inhibited. CONCLUSIONS: These data indicate that integrin beta3-FAK signaling modulates OPN-induced VSMC migration during neointimal formation.
BACKGROUND:Osteopontin (OPN) promotes the migration and adhesion of vascular smooth muscle cells (VSMCs) through cell surface receptor, integrin beta3. In order to elucidate the signaling pathway by which OPN is involved in neointimal formation, we focused on integrin beta3-focal adhesion kinase (FAK) upon VSMC migration. METHODS: The integrin beta3 and FAK expression in VSMC and in neointima was detected by Western blot and immunohistochemistry staining. FAK phosphorylation induced by OPN was verified using a linear OPN 13 peptide containing RGD motif and anti-OPN antibody. The role of integrin beta3-FAK pathway in VSMC adhesion and migration induced with OPN was tested by the overexpression of FAK-related nonkinase and integrin beta3 cytoplasmic domain. RESULTS: The results showed that OPN increased integrin beta3 expression and induced rapid and transient FAK phosphorylation. Inhibition of the phosphorylation of FAK significantly suppressed VSMC migration induced by OPN. Similarly, blockade of the interaction of integrin beta3 with OPN inhibited VSMC adhesion induced by OPN. The experiment, in vivo, demonstrated that OPN expression level was consistent with neointimal thickening. Administration of anti-OPN antibody for blocking OPN function suppressed integrin beta3 and FAK expression induced by balloon injury, and neointimal thickening was inhibited. CONCLUSIONS: These data indicate that integrin beta3-FAK signaling modulates OPN-induced VSMC migration during neointimal formation.
Authors: Teresa Cascella; Yashwanth Radhakrishnan; Laura A Maile; Walker H Busby; Katherine Gollahon; Annamaria Colao; David R Clemmons Journal: Endocrinology Date: 2010-09-29 Impact factor: 4.736
Authors: Kyuho Jeong; Jung-Hyun Kim; James M Murphy; Hyeonsoo Park; Su-Jeong Kim; Yelitza A R Rodriguez; Hyunkyung Kong; Chungsik Choi; Jun-Lin Guan; Joan M Taylor; Thomas M Lincoln; William T Gerthoffer; Jun-Sub Kim; Eun-Young Erin Ahn; David D Schlaepfer; Ssang-Taek Steve Lim Journal: Circ Res Date: 2019-05-17 Impact factor: 17.367
Authors: Guohong Li; Rong Jin; Russell A Norris; Lin Zhang; Shiyong Yu; Fusheng Wu; Roger R Markwald; Anil Nanda; Simon J Conway; Susan S Smyth; D Neil Granger Journal: Atherosclerosis Date: 2009-07-30 Impact factor: 5.162