| Literature DB >> 1786856 |
M Sarikaputi1, M Morimatsu, B Syuto, M Saito, M Naiki.
Abstract
1. A new purification procedure was started with salting-out fractionation of serum proteins at 45-75% saturated ammonium sulfate concentration, followed by HE agarose affinity chromatography by which calcium-dependently bound CRP and SAP were purely eluted with EDTA-containing buffer. 2. Pure CRP and SAP were finally separated by DEAE-5PW HPLC. 3. This procedure gave recovery of 15 and 26%, and fold purification of 2650 and 2400 for CRP and SAP, respectively. 4. Each subunit of CRP and SAP had one intrasubunit disulfide bond, determined by reduction and carboxymethylation.Entities:
Mesh：
Substances：
Year: 1991 PMID： 1786856 DOI： 10.1016/0020-711x(91)90155-g
Source DB: PubMed Journal: Int J Biochem ISSN： 0020-711X