Literature DB >> 1785693

A single-reaction method for DNA sequence determination.

R Negri1, G Costanzo, E Di Mauro.   

Abstract

A chemical method for the determination of DNA sequence is presented. Heating of DNA, labeled at a single 3' extremity, in the presence of formamide results in efficient cleavage of phosphodiester bonds 3' of A, G, and C residues. The relative efficiency is A = G greater than C. The bias between A and G is solved by a simple pretreatment (photoreaction in methylene blue) followed by heating in formamide. The entire procedure does not require any intermediate purification step or handling of hazardous chemicals and allows determination of the sequence on only two electrophoretic lanes. Its simplicity and rapidity favor automatization.

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Year:  1991        PMID： 1785693     DOI： 10.1016/0003-2697(91)90409-m

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  Sequencing two DNA templates in five channels by digital compression.

Authors:  M Nelson; Y Zhang; D L Steffens; R Grabherr; J L Van Etten
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-01       Impact factor: 11.205

2.  One-lane chemical sequencing of PCR amplified DNA: the use of terminal transferase and of the base analogue inosine.

Authors:  E Di Mauro; G Costanzo; R Negri
Journal:  Nucleic Acids Res       Date:  1994-09-11       Impact factor: 16.971

3.  Chemical method for DNA sequence determination from the 5'-extremity on PCR amplified fragments.

Authors:  R Negri; S Ferraboli; S Barlati; E Di Mauro
Journal:  Nucleic Acids Res       Date:  1994-01-11       Impact factor: 16.971

4.  Two distinct factors bind to the rabbit uteroglobin TATA-box region and are required for efficient transcription.

Authors:  J Klug; S Knapp; I Castro; M Beato
Journal:  Mol Cell Biol       Date:  1994-09       Impact factor: 4.272

5.  Two-photon fluorescence cross-correlation spectroscopy as a potential tool for high-throughput screening of DNA repair activity.

Authors:  Maddalena Collini; Michele Caccia; Giuseppe Chirico; Flavia Barone; Eugenia Dogliotti; Filomena Mazzei
Journal:  Nucleic Acids Res       Date:  2005-10-21       Impact factor: 16.971
  5 in total

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