Yingying Gu1, Qingge Li. 1. Molecular Diagnostics Laboratory, Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
Abstract
OBJECTIVES: This study aimed to develop and validate a rapid, accurate method for aldehyde dehydrogenase-2 gene (ALDH2) genotyping. DESIGN AND METHODS: We reported a single-tube, real-time PCR method for ALDH2 allele detection using two displacing probes. RESULTS: The genotyping results of 136 human genomic DNA samples indicated 100% concordance between real-time PCR method and the PCR-RFLP analysis. CONCLUSIONS: The developed method could be used for routine clinical testing and high throughput genetic screening of ALDH2.
OBJECTIVES: This study aimed to develop and validate a rapid, accurate method for aldehyde dehydrogenase-2 gene (ALDH2) genotyping. DESIGN AND METHODS: We reported a single-tube, real-time PCR method for ALDH2 allele detection using two displacing probes. RESULTS: The genotyping results of 136 human genomic DNA samples indicated 100% concordance between real-time PCR method and the PCR-RFLP analysis. CONCLUSIONS: The developed method could be used for routine clinical testing and high throughput genetic screening of ALDH2.