Mahmoud S Ali1, J A Wilson, M Bennett, Jeffrey P Pearson. 1. Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, UK. msamomar@yahoo.co.uk
Abstract
CONCLUSION: Membrane-bound mucin MUC4 represents the predominant mucin expressed in the adenoid epithelium followed by MUC5AC (gel-forming mucin). This may suggest that membrane-bound mucins could be involved in pathogen binding and immunological stimulation. OBJECTIVES: The aim of this study was to investigate mucin expression in hypertrophic adenoids. MATERIALS AND METHODS: Adenoidal samples were obtained from 12 children. The expression of eight mucin genes, MUC1-4, MUC5AC, 5B, 6 and 7 was studied by in situ hybridization utilizing digoxigenin-labelled oligonucleotide probes. RESULTS: The dominant mucin genes were MUC4, 3 and 5AC, while MUC1, 2, 5B and 7 were sparsely expressed and MUC6 was not expressed. Expression patterns were very different from those in the upper airways. Most samples expressed two membrane-bound mucins (MUC4 and 3) and one secretory mucin (MUC5AC).
CONCLUSION: Membrane-bound mucinMUC4 represents the predominant mucin expressed in the adenoid epithelium followed by MUC5AC (gel-forming mucin). This may suggest that membrane-bound mucins could be involved in pathogen binding and immunological stimulation. OBJECTIVES: The aim of this study was to investigate mucin expression in hypertrophic adenoids. MATERIALS AND METHODS: Adenoidal samples were obtained from 12 children. The expression of eight mucin genes, MUC1-4, MUC5AC, 5B, 6 and 7 was studied by in situ hybridization utilizing digoxigenin-labelled oligonucleotide probes. RESULTS: The dominant mucin genes were MUC4, 3 and 5AC, while MUC1, 2, 5B and 7 were sparsely expressed and MUC6 was not expressed. Expression patterns were very different from those in the upper airways. Most samples expressed two membrane-bound mucins (MUC4 and 3) and one secretory mucin (MUC5AC).
Authors: David Hsu; Airie Kim; Tomas Ganz; Jeffrey D Suh; Marilene Wang; Tara J Wu; Jivianne T Lee Journal: Otolaryngol Head Neck Surg Date: 2020-08-04 Impact factor: 3.497