Literature DB >> 1784817

Levansucrase: a tool to study protein secretion in Bacillus subtilis.

V Nagarajan1, T V Borchert.   

Abstract

The Bacillus amyloliquefaciens levansucrase gene (sacB[BamP]) was engineered in such a way that a heterologous gene could be inserted between the second and third codon of the mature levansucrase. Extracellular levansucrase activity was detected only when the heterologous protein was secreted into the growth medium. A positive selection system to isolate suppressors of signal sequence mutants in Bacillus subtilis has been developed based on the secretion of levansucrase.

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Year:  1991        PMID： 1784817     DOI： 10.1016/0923-2508(91)90056-g

Source DB:  PubMed          Journal:  Res Microbiol        ISSN： 0923-2508            Impact factor:   3.992


  6 in total

1.  Direct selection of cloned DNA in Bacillus subtilis based on sucrose-induced lethality.

Authors:  M G Bramucci; V Nagarajan
Journal:  Appl Environ Microbiol       Date:  1996-11       Impact factor: 4.792

2.  Chloramphenicol acetyltransferase, a cytoplasmic protein is incompatible for export from Bacillus subtilis.

Authors:  M W Chen; V Nagarajan
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

3.  Signal peptide and propeptide optimization for heterologous protein secretion in Lactococcus lactis.

Authors:  Y Le Loir; S Nouaille; J Commissaire; L Brétigny; A Gruss; P Langella
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

4.  Fructan Accumulation and Sucrose Metabolism in Transgenic Maize Endosperm Expressing a Bacillus amyloliquefaciens SacB Gene.

Authors:  P. G. Caimi; L. M. McCole; T. M. Klein; P. S. Kerr
Journal:  Plant Physiol       Date:  1996-02       Impact factor: 8.340

5.  The roles of signal peptide and mature protein in RNase (barnase) export from Bacillus subtilis.

Authors:  M Chen; V Nagarajan
Journal:  Mol Gen Genet       Date:  1993-06

6.  Characterization of the secretion efficiency of a plant signal peptide in Bacillus subtilis.

Authors:  J Ribbe; V Nagarajan
Journal:  Mol Gen Genet       Date:  1992-11
  6 in total

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