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Literature DB >> 17838129

Induction and Isolation of Auxotrophic Mutants in Somatic Cell Cultures of Nicotiana tabacum.

Abstract

A method for the selection of auxotrophic mutants from somatic cell cultures of Nicotiana tabacum depends upon the incorporation of 5-bromodeoxyuridine into the DNA of wild-type cells and upon its lack of incorporation into the DNA of auxotrophic cells. There may be more than one functional copy of essential genes in the haploid genome of Nicotiana tabacum.

Entities: Chemical Species

Year: 1970 PMID： 17838129 DOI： 10.1126/science.168.3930.487

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

25 in total

1. Characterization of the altered anthranilate synthase in 5-methyltryptophan-resistant rice mutants.

Authors: D S Kim; I S Lee; C S Jang; S-Y Kang; Y W Seo
Journal: Plant Cell Rep Date: 2005-03-18 Impact factor: 4.570

2. Parasexual interspecific plant hybridization.

Authors: P S Carlson; H H Smith; R D Dearing
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

3. The use of protoplasts for genetic research.

Authors: P S Carlson
Journal: Proc Natl Acad Sci U S A Date: 1973-02 Impact factor: 11.205

4. Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus : II. Auxotrophic and temperature-sensitive clones.

Authors: C Gebhardt; V Schnebli; P J King
Journal: Planta Date: 1981-10 Impact factor: 4.116

5. Advances in somatic cell genetics of higher plants - the protoplast approach in basic studies on mutagenesis and isolation of biochemical mutants.

Authors: I Negrutiu; M Jacobs; M Caboche
Journal: Theor Appl Genet Date: 1984-02 Impact factor: 5.699

Induction and Isolation of Auxotrophic Mutants in Somatic Cell Cultures of Nicotiana tabacum.

1. Characterization of the altered anthranilate synthase in 5-methyltryptophan-resistant rice mutants.

2. Parasexual interspecific plant hybridization.

3. The use of protoplasts for genetic research.

4. Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus : II. Auxotrophic and temperature-sensitive clones.

5. Advances in somatic cell genetics of higher plants - the protoplast approach in basic studies on mutagenesis and isolation of biochemical mutants.

6. Partial characterization of two stable auxotrophic cell strains of Datura innoxia Mill.

7. Induction of haploidy from pollen grains in angiosperms - the current status.

8. Biotechnological applications of plant cells.

9. A method for high-frequency intergeneric fusion of plant protoplasts.

10. A mutation causing proline requirement in Zea mays.