OBJECTIVE: The objective of this study was to describe the effects of short-term dietary exposure of bovine lactoferrin (Lf) on intestinal lymphocyte apoptosis and expression of tumor necrosis factor-alpha (TNF-alpha) in healthy mice. METHODS: Female C57BL/6 mice were randomly assigned to three treatment groups: 0% Lf (n = 16), 0.2% Lf (n = 16), and 2.0% Lf (n = 15). Bovine Lf was administered orally, as part of the diet, for 4 consecutive days. Intestinal lymphocytes (ILs) were isolated and analyzed for percentages of CD4, CD8, apoptotic CD4, and apoptotic CD8 cells using flow cytometry. Pro- (caspase-3) and anti- (Bcl-2) apoptotic protein expressions and TNF-alpha expression in ILs were determined by Western blotting. RESULTS: There were significant increases in the percentages of CD4 (P = 0.02) and apoptotic CD4 (P = 0.02) ILs in bovine Lf-fed compared with control mice. Percentages of CD8 and apoptotic CD8 cells and expression of caspase-3 and Bcl-2 in ILs did not differ significantly by diet group. In contrast, the expression of TNF-alpha was significantly lower in Lf-fed versus control mice (P = 0.01). CONCLUSION: Short-term dietary Lf decreased TNF-alpha expression in ILs and increased apoptosis of CD4 ILs in healthy mice.
OBJECTIVE: The objective of this study was to describe the effects of short-term dietary exposure of bovinelactoferrin (Lf) on intestinal lymphocyte apoptosis and expression of tumor necrosis factor-alpha (TNF-alpha) in healthy mice. METHODS: Female C57BL/6 mice were randomly assigned to three treatment groups: 0% Lf (n = 16), 0.2% Lf (n = 16), and 2.0% Lf (n = 15). Bovine Lf was administered orally, as part of the diet, for 4 consecutive days. Intestinal lymphocytes (ILs) were isolated and analyzed for percentages of CD4, CD8, apoptotic CD4, and apoptotic CD8 cells using flow cytometry. Pro- (caspase-3) and anti- (Bcl-2) apoptotic protein expressions and TNF-alpha expression in ILs were determined by Western blotting. RESULTS: There were significant increases in the percentages of CD4 (P = 0.02) and apoptotic CD4 (P = 0.02) ILs in bovine Lf-fed compared with control mice. Percentages of CD8 and apoptotic CD8 cells and expression of caspase-3 and Bcl-2 in ILs did not differ significantly by diet group. In contrast, the expression of TNF-alpha was significantly lower in Lf-fed versus control mice (P = 0.01). CONCLUSION: Short-term dietary Lf decreased TNF-alpha expression in ILs and increased apoptosis of CD4 ILs in healthy mice.
Authors: Gerardo Ramírez-Rico; Maria Elisa Drago-Serrano; Nidia León-Sicairos; Mireya de la Garza Journal: Front Pharmacol Date: 2022-02-21 Impact factor: 5.810
Authors: Víctor A Solarte; Jaiver E Rosas; Zuly J Rivera; Martha L Arango-Rodríguez; Javier E García; Jean-Paul Vernot Journal: Biomed Res Int Date: 2015-11-02 Impact factor: 3.411