Literature DB >> 1782145

ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance.

A Kimura1, F Matsumura, K Sodesaki, M Osawa, H Ikeda, S Yasuda, T Tsuji.   

Abstract

Practical methods were developed for ABO blood grouping of semen from mixed body fluids. An monoclonal antibody (P6-5H) which recognizes a tissue-specific epitope on a seminal ABO blood group substance (alpha 2-seminoglycoprotein) was used as a solid phase antibody for selective capture of the seminal ABO blood group substance. ABO blood group epitopes of secretor and non-secretor semen were detected in dilutions of 8 x 10(3)-3.2 x 10(4) and 8 x 10(3)-fold, respectively, by sandwich ELISA. ABO blood group epitopes were also detected in dilutions up to 4 x 10(3)-fold, irrespective of secretor status, by the sandwich absorption-elution test.

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Year:  1991        PMID： 1782145     DOI： 10.1007/bf01369580

Source DB:  PubMed          Journal:  Int J Legal Med        ISSN： 0937-9827            Impact factor:   2.686


  7 in total

1.  ABO blood grouping of saliva from mixed body fluids by sandwich methods using monoclonal antibodies to tissue specific epitopes on blood group substance in saliva.

Authors:  A Kimura; F Matsumura; K Sodesaki; T Tsuji
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

2.  [Immunochemical studies of a glycoprotein (alpha 2-seminoglycoprotein; alpha 2-SGP) in seminal plasma--forensic immunological studies of body fluids and secretions, report 33].

Authors:  R Tsuda; Y Ito; M Hara
Journal:  Nihon Hoigaku Zasshi       Date:  1988-02

3.  [Detection of ABO blood group of seminal stains mixed with vaginal secretion or blood by a method using NCF coated with anti alpha 2-SGP serum--forensic immunological studies of body fluids and secretions, report XXXVI].

Authors:  H Iki; H Minamitake; R Tsuda; M Hara
Journal:  Nihon Hoigaku Zasshi       Date:  1988-12

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Production and characterization of monoclonal antibodies against tissue specific epitopes on ABO blood group substances in saliva.

Authors:  A Kimura; F Matsumura; K Sodesaki; T Tsuji
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

7.  Cytochemical detection of ABH antigens in human body fluids.

Authors:  B Brinkmann; G Kernbach; S Rand
Journal:  Z Rechtsmed       Date:  1986
  7 in total
  2 in total

1.  Brain tissue identification based on myosin heavy chain isoforms.

Authors:  A Kimura; H Ikeda; S Yasuda; K Yamaguchi; T Tsuji
Journal:  Int J Legal Med       Date:  1995       Impact factor: 2.686

2.  ABO blood grouping of bloodstains by sandwich ELISA using monoclonal antibody specific for human red cell band 3.

Authors:  A Kimura; T Uda; S Nakashima; H Ikeda; S Yasuda; M Osawa; T Tsuji
Journal:  Int J Legal Med       Date:  1993       Impact factor: 2.686
  2 in total

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