Literature DB >> 17805725

Separation of cholesterol-desmosterol acetates by thin-layer and column chromatography on silica gel G-silver nitrate.

G Galli1, E G Paoletti.   

Abstract

A chromatographic method, using columns packed with Celite-Silica Gel G impregnated with silver nitrate, has been developed for the separation of cholesterol from desmosterol (24-dehydrocholesterol).The system employed allows a complete separation of the two sterols as acetates, as determined by gas-liquid chromatography and thin-layer chromatography.The recovery of pure cholesterol and desmosterol is quantitative.

Entities:  

Year:  1967        PMID： 17805725     DOI： 10.1007/BF02532004

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  9 in total

1.  THIN-LAYER CHROMATOGRAPHY OF STEROLS AND STEROIDS.

Authors:  J AVIGAN; D S GOODMAN; D STEINBERG
Journal:  J Lipid Res       Date:  1963-01       Impact factor: 5.922

2.  SEPARATION OF CHOLESTEROL AND DEMOSTEROL BY THIN-LAYER CHROMATOGRAPHY.

Authors:  L WOLFMAN; B A SACHS
Journal:  J Lipid Res       Date:  1964-01       Impact factor: 5.922

3.  Studies of cholesterol biosynthesis. I. The identification of desmosterol in serum and tissues of animals and man treated with MER-29.

Authors:  J AVIGAN; D STEINBERG; H E VROMAN; M J THOMPSON; E MOSETTIG
Journal:  J Biol Chem       Date:  1960-11       Impact factor: 5.157

4.  SEPARATION OF CHOLESTEROL FROM ITS COMPANIONS, CHOLESTANOL AND DELTA-7-CHOLESTENOL, BY THIN-LAYER CHROMATOGRAPHY.

Authors:  A S TRUSWELL; W D MITCHELL
Journal:  J Lipid Res       Date:  1965-07       Impact factor: 5.922

5.  Thin-layer chromatography of sterols.

Authors:  R D BENNETT; E HEFTMANN
Journal:  J Chromatogr       Date:  1962-11

6.  A study of retentiontime relationships in gas chromatography in terms of the structure of steroids.

Authors:  W J VANDENHEUVEL; E C HORNING
Journal:  Biochim Biophys Acta       Date:  1962-11-05

7.  Metabolism of cholesterol in the chick embryo. II. Isolation and chemical nature of two companion sterols.

Authors:  W M STOKES; W A FISH; F C HICKEY
Journal:  J Biol Chem       Date:  1956-05       Impact factor: 5.157

8.  A new method for the direct determination of serum cholesterol.

Authors:  A ZLATKIS; B ZAK; A J BOYLE
Journal:  J Lab Clin Med       Date:  1953-03

9.  THE ANALYSIS OF MIXTURES OF ANIMAL AND VEGETABLE FATS. V. SEPARATION OF STEROL ACETATES BY THIN-LAYER CHROMATOGRAPHY IN REVERSED-PHASE SYSTEMS AND ON SILICA GEL G-SILVER NITRATE LAYERS.

Authors:  J W COPIUS-PEEREBOOM; H W BEEKES
Journal:  J Chromatogr       Date:  1965-01
  9 in total
  1 in total

1.  Some pathways and mechanisms in lanosterol-cholesterol conversion in mammalian tissues.

Authors:  R Paoletti; G Galli; E Grossi Paoletti; A Fiecchi; A Scala
Journal:  Lipids       Date:  1971-02       Impact factor: 1.880
  1 in total

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