| Literature DB >> 1780169 |
A Achbarou1, O Mercereau-Puijalon, A Sadak, B Fortier, M A Leriche, D Camus, J F Dubremetz.
Abstract
The biosynthesis and fate of 4 different dense granule proteins of Toxoplasma gondii were studied with 3 monoclonal antibodies raised against tachyzoites and 1 polyclonal antibody raised against a recombinant protein. These proteins have the following molecular weights: 27 kDa (GRA 1), 28 kDa (GRA 2), 30 kDa (GRA 3) and 40 kDa (GRA 4). All four proteins were found in dense granules by immunoelectron microscopy; in T. gondii-infected cells, they were found in the vacuolar network but, in addition, GRA 3 was also detected on the parasitophorous vacuole membrane. Therefore, dense granule contents undergo differential targeting when exocytosed in the parasitophorous vacuole. Metabolic labelling and immunoprecipitation showed that GRA 2 and GRA 3 were processed from lower molecular weight precursors, and that GRA 2 and GRA 4 incorporated [3H] glucosamine and are thus likely to be glycosylated.Entities:
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Year: 1991 PMID： 1780169 DOI： 10.1017/s0031182000059837
Source DB: PubMed Journal: Parasitology ISSN： 0031-1820 Impact factor: 3.234