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Literature DB >> 1780025

Structure and function of L-glutamate decarboxylase.

J Y Wu¹, W M Huang, L Reed-Fourquet, J Bao, B Nathan, E Wu, W H Tsai.

Abstract

Membrane bound L-glutamate decarboxylase (GAD) has been solubilized and partially purified from hog brain. The solubilized GAD appears to exist in two forms, alpha and beta, differing in their size and electrophoretic mobility. The alpha form has similar mobility as that of the soluble GAD in 7.5% and 5-25% gradient polyacrylamide gel electrophoresis suggesting that they are similar in size and charge. In addition, gene encoding for mouse brain GAD has been cloned and characterized. Mouse brain GAD cDNA consists of two DNA fragments with 1.6 and 1.0 Kb. The 1.6 and 1.0 Kb fragments contain 1657 and 974 bP, respectively. The significance of multiple forms of GAD is also discussed.

Entities: Chemical Species

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Year: 1991 PMID： 1780025 DOI： 10.1007/bf00966085

Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996

25 in total

1. A rapid method for assaying enzymes whose substrates and products differ by charge. Application to brain L-glutamate decarboxylase.

Authors: O Chude; J Y Wu
Journal: J Neurochem Date: 1976-07 Impact factor: 5.372

3. A membrane form of brain L-glutamate decarboxylase: identification, isolation, and its relation to insulin-dependent mellitus.

Authors: B Nathan; J Bao; C C Hsu; P Aguilar; R Wu; M Yarom; C Y Kuo; J Y Wu
Journal: Proc Natl Acad Sci U S A Date: 1994-01-04 Impact factor: 11.205

3 in total

Structure and function of L-glutamate decarboxylase.

1. A rapid method for assaying enzymes whose substrates and products differ by charge. Application to brain L-glutamate decarboxylase.

2. Purification and characterization of a benzodiazepine-like substance from mammalian brain.

3. Regional distribution of the GABAA/benzodiazepine receptor (alpha subunit) mRNA in rat brain.

4. Kinetically different, multiple forms of glutamate decarboxylase in rat brain.

5. Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase.

6. Efficient isolation of genes by using antibody probes.

7. Properties of brain L-glutamate decarboxylase: inhibition studies.

8. Comparison of the structures of L-glutamate decarboxylases from human and rat brains.

9. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

10. Two forms of rat brain glutamic acid decarboxylase differ in their dependence on free pyridoxal phosphate.

1. Isolation and characterization of endogenous modulators for GABA system.

2. Cloning and characterization of myr 6, an unconventional myosin of the dilute/myosin-V family.

3. A membrane form of brain L-glutamate decarboxylase: identification, isolation, and its relation to insulin-dependent mellitus.