Literature DB >> 17773042

Biosynthesis of agr-Ecdysone by Prothoracic Glands in vitro.

H Chino, S Sakurai, T Ohtaki, N Ikekawa, H Miyazaki, M Ishibashi, H Abuki.   

Abstract

An in vitro study in which isolated prothoracic glands of the Bombyx silkworm were cultured has provided definite evidence that the prothoracic gland is the site where molting hormone is synthesized. The hormone behaved very similarly to free ecdysone on thin-layer chromatography. Analysis by liquid chromatography and mass fragmentography revealed that the hormone is identical with alpha-ecdysone.

Entities:  

Year:  1974        PMID： 17773042     DOI： 10.1126/science.183.4124.529

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  20 in total

1.  Alpha- and beta-ecdysone levels in insect haemolymph: correlation with developmental events.

Authors:  R Lafont; B Mauchamp; J L Pennetier; P Tarroux; L de Hys; J P Delbecque
Journal:  Experientia       Date:  1975-10-15

2.  Epidermis as the source of ecdysone in an argasid tick.

Authors:  X X Zhu; J H Oliver; E M Dotson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-01       Impact factor: 11.205

3.  RNA biosynthesis in isolated prothoracic glands of Tenebrio molitor in vitro.

Authors:  V Weber; H Emmerich
Journal:  Experientia       Date:  1976-12-15

4.  Improved conditions for culture of biosynthetically active cockroach corpora allata.

Authors:  G L Holbrook; A S Chiang; C Schal
Journal:  In Vitro Cell Dev Biol Anim       Date:  1997-06       Impact factor: 2.416

5.  Prothoracic gland synthesis of 3-dehydroecdysone and its hemolymph 3 beta-reductase mediated conversion to ecdysone in representative insects.

Authors:  S Kiriishi; D B Rountree; S Sakurai; L I Gilbert
Journal:  Experientia       Date:  1990-07-15

6.  [Regeneration, differentiation and moulting hormones in the cockroach embryo cultured in vitro].

Authors:  Françoise Bullière; Désiré Bullière
Journal:  Wilehm Roux Arch Dev Biol       Date:  1977-09

7.  The in vitro induction of puffing in salivary glands of the mutantl (2)gl ofDrosophila melanogaster by ecdysone.

Authors:  G P Richards
Journal:  Wilehm Roux Arch Dev Biol       Date:  1976-12

8.  Secretion of alpha-ecdysone by crab Y-organs in vitro.

Authors:  E S Chang; J D O'Connor
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

9.  Electron microscopical-immunocytochemical evidence of ecdysteroids in the prothoracic gland of Galleria mellonella.

Authors:  H Birkenbeil; M Eckert; M Gersch
Journal:  Cell Tissue Res       Date:  1979-08       Impact factor: 5.249

10.  Insect steroid metabolism.

Authors:  J A Svoboda; M J Thompson; W E Robbins; J N Kaplanis
Journal:  Lipids       Date:  1978-10       Impact factor: 1.880
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