Literature DB >> 17768353

Crystallization and preliminary crystallographic analysis of an esterase with a novel domain from the hyperthermophile Thermotoga maritima.

Lei Sun1, Mark Levisson, Sjon Hendriks, Twan Akveld, Servé W M Kengen, Bauke W Dijkstra, John van der Oost.   

Abstract

A predicted esterase (EstA) with an unusual new domain from the hyperthermophilic bacterium Thermotoga maritima has been cloned and overexpressed in Escherichia coli. The purified protein was crystallized by the hanging-drop vapour-diffusion technique in the presence of lithium sulfate and polyethylene glycol 8000. Selenomethionine-substituted EstA crystals were obtained under the same conditions and three different-wavelength data sets were collected to 2.6 A resolution. The crystal belongs to space group H32, with unit-cell parameters a = b = 130.2, c = 306.2 A. There are two molecules in the asymmetric unit, with a V(M) of 2.9 A3 Da(-1) and 58% solvent content.

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Year:  2007        PMID： 17768353      PMCID： PMC2376313          DOI： 10.1107/S174430910703953X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  7 in total

1.  Purification, crystallization and preliminary crystallographic analysis of phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  Jasper Akerboom; Andrew P Turnbull; David Hargreaves; Martin Fisher; Daniel de Geus; Svetlana E Sedelnikova; John M Berrisford; Patrick J Baker; Corne H Verhees; John van der Oost; David W Rice
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-09-19

2.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

Review 3.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

Authors:  S F Altschul; T L Madden; A A Schäffer; J Zhang; Z Zhang; W Miller; D J Lipman
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

Review 4.  Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.

Authors:  K E Jaeger; B W Dijkstra; M T Reetz
Journal:  Annu Rev Microbiol       Date:  1999       Impact factor: 15.500

5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

6.  Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima.

Authors:  K E Nelson; R A Clayton; S R Gill; M L Gwinn; R J Dodson; D H Haft; E K Hickey; J D Peterson; W C Nelson; K A Ketchum; L McDonald; T R Utterback; J A Malek; K D Linher; M M Garrett; A M Stewart; M D Cotton; M S Pratt; C A Phillips; D Richardson; J Heidelberg; G G Sutton; R D Fleischmann; J A Eisen; O White; S L Salzberg; H O Smith; J C Venter; C M Fraser
Journal:  Nature       Date:  1999-05-27       Impact factor: 49.962

7.  Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima.

Authors:  Mark Levisson; John van der Oost; Servé W M Kengen
Journal:  FEBS J       Date:  2007-04-27       Impact factor: 5.542
  7 in total
  2 in total

1.  Characterization of a novel thermostable esterase from Thermus scotoductus SA-01: evidence of a new family of lipolytic esterases.

Authors:  Erika M du Plessis; Eldie Berger; Therese Stark; Maureen E Louw; Daniel Visser
Journal:  Curr Microbiol       Date:  2009-12-05       Impact factor: 2.188

Review 2.  Carboxylic ester hydrolases from hyperthermophiles.

Authors:  Mark Levisson; John van der Oost; Servé W M Kengen
Journal:  Extremophiles       Date:  2009-06-21       Impact factor: 2.395
  2 in total

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