Literature DB >> 17768345

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217.

Jun Wada1, Ryuichiro Suzuki, Shinya Fushinobu, Motomitsu Kitaoka, Takayoshi Wakagi, Hirofumi Shoun, Hisashi Ashida, Hidehiko Kumagai, Takane Katayama, Kenji Yamamoto.   

Abstract

A recombinant galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9-6.4 and 20-22%(v/v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 A for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 A for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 A resolution, respectively.

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Year:  2007        PMID: 17768345      PMCID: PMC2376316          DOI: 10.1107/S1744309107036263

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  22 in total

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4.  Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1, complexed with an alginate tetrasaccharide at 1.6-A resolution.

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Review 9.  Oligosaccharides in human milk: structural, functional, and metabolic aspects.

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  16 in total

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Journal:  J Biol Chem       Date:  2011-08-09       Impact factor: 5.157

2.  Syntheses of mucin-type O-glycopeptides and oligosaccharides using transglycosylation and reverse-hydrolysis activities of Bifidobacterium endo-alpha-N-acetylgalactosaminidase.

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Journal:  Glycoconj J       Date:  2009-06-27       Impact factor: 2.916

3.  Human milk glycobiome and its impact on the infant gastrointestinal microbiota.

Authors:  Angela M Zivkovic; J Bruce German; Carlito B Lebrilla; David A Mills
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4.  Bifidobacterium breve UCC2003 Employs Multiple Transcriptional Regulators To Control Metabolism of Particular Human Milk Oligosaccharides.

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Review 5.  Nursing our microbiota: molecular linkages between bifidobacteria and milk oligosaccharides.

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6.  α-N-acetylgalactosaminidase from infant-associated bifidobacteria belonging to novel glycoside hydrolase family 129 is implicated in alternative mucin degradation pathway.

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7.  Characterization of three beta-galactoside phosphorylases from Clostridium phytofermentans: discovery of d-galactosyl-beta1->4-l-rhamnose phosphorylase.

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8.  Distribution of in vitro fermentation ability of lacto-N-biose I, a major building block of human milk oligosaccharides, in bifidobacterial strains.

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9.  Bifidobacterium bifidum lacto-N-biosidase, a critical enzyme for the degradation of human milk oligosaccharides with a type 1 structure.

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Review 10.  Bifidobacterial enzymes involved in the metabolism of human milk oligosaccharides.

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