| Literature DB >> 17768345 |
Jun Wada1, Ryuichiro Suzuki, Shinya Fushinobu, Motomitsu Kitaoka, Takayoshi Wakagi, Hirofumi Shoun, Hisashi Ashida, Hidehiko Kumagai, Takane Katayama, Kenji Yamamoto.
Abstract
A recombinant galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9-6.4 and 20-22%(v/v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 A for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 A for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 A resolution, respectively.Entities:
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Year: 2007 PMID: 17768345 PMCID: PMC2376316 DOI: 10.1107/S1744309107036263
Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN: 1744-3091