Comparative roles of acetylcholine and noradrenaline in controlling the spontaneous activity of cortical neurons.

The effects of acetylcholine and noradrenaline on the spike activity of neurons recorded in guinea pig parietal cortex slices were studied. Iontophoretic application of these two neurotransmitters to cortical neurons induced similar responses consisting of slowly developing and prolonged increases in spike activity. Differences in the temperature sensitivity of responses to acetylcholine and noradrenaline were identified. When the incubation medium temperature was increased from 32-34 degrees C to 35-36 degrees C, the effects of acetylcholine on neuron spike activity increased sharply, with the result that neurons which showed no spontaneous activity at 32-34 degrees C became sensitive to acetylcholine. The temperature-dependent increases in the extent of responses to acetylcholine were accompanied by stable increases in the level of spontaneous activity. Responses to application of noradrenaline showed no significant change when the temperature increased from 32-34 degrees C to 35-36 degrees C. Since neuron responses to the iontophoretic application of glutamate, the major excitatory neurotransmitter in the cortex, remained constant over this range of temperatures, the data obtained here lead to the conclusion that acetylcholine is the main regulator of the level of spontaneous activity of cortical neurons.