Literature DB >> 17761480

Sequential multiplex PCR for identifying pneumococcal capsular serotypes from South-Saharan African clinical isolates.

Luis Morais1, Maria da Glória Carvalho2, Anna Roca3,1, Brendan Flannery2, Inacio Mandomando4,1, Montserrat Soriano-Gabarró2, Betuel Sigauque4,1, Pedro Alonso3,1, Bernard Beall2.   

Abstract

A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17761480     DOI： 10.1099/jmm.0.47346-0

Source DB:  PubMed          Journal:  J Med Microbiol        ISSN： 0022-2615            Impact factor:   2.472


  28 in total

1.  From Quellung to multiplex PCR, and back when needed, in pneumococcal serotyping.

Authors:  Lotta Siira; Tarja Kaijalainen; Lotte Lambertsen; Moon H Nahm; Maija Toropainen; Anni Virolainen
Journal:  J Clin Microbiol       Date:  2012-06-12       Impact factor: 5.948

2.  Molecular epidemiological investigation to determine the source of a fatal case of serotype 22F pneumococcal meningitis.

Authors:  Juliana Lamaro-Cardoso; Ana Paula S de Lemos; Maria da Glória Carvalho; Fabiana Cristina Pimenta; Alexis Roundtree; Lorena Motta; Maria Aparecida Vieira; Sabrina Sgambatti; Licia Kamila Thörn; Vicente Pessoa-Junior; Ruth Minamisava; Lee H Harrison; Bernard W Beall; Maria Cristina de Cunto Brandileone; Ana Lucia Andrade
Journal:  J Med Microbiol       Date:  2012-01-27       Impact factor: 2.472

3.  Rarely occurring 19A-like cps locus from a serotype 19F pneumococcal isolate indicates continued need of serology-based quality control for PCR-based serotype determinations.

Authors:  Fabiana C Pimenta; Robert E Gertz; Alexis Roundtree; Jigui Yu; Moon H Nahm; Ryan R McDonald; Maria da Gloria Carvalho; Bernard W Beall
Journal:  J Clin Microbiol       Date:  2009-05-13       Impact factor: 5.948

4.  Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid.

Authors:  Berthe-Marie Njanpop Lafourcade; Oumarou Sanou; Mark van der Linden; Natalia Levina; Meryem Karanfil; Seydou Yaro; Tsidi A Tamekloe; Judith E Mueller
Journal:  J Clin Microbiol       Date:  2009-12-09       Impact factor: 5.948

5.  Rapid and easy identification of capsular serotypes of Streptococcus pneumoniae by use of fragment analysis by automated fluorescence-based capillary electrophoresis.

Authors:  Laura Selva; Eva del Amo; Pedro Brotons; Carmen Muñoz-Almagro
Journal:  J Clin Microbiol       Date:  2012-08-08       Impact factor: 5.948

6.  Revisiting pneumococcal carriage by use of broth enrichment and PCR techniques for enhanced detection of carriage and serotypes.

Authors:  Maria da Gloria Carvalho; Fabiana C Pimenta; Delois Jackson; Alexis Roundtree; Yusra Ahmad; Eugene V Millar; Katherine L O'Brien; Cynthia G Whitney; Adam L Cohen; Bernard W Beall
Journal:  J Clin Microbiol       Date:  2010-03-10       Impact factor: 5.948

7.  Serotyping of Streptococcus pneumoniae isolates from nasopharyngeal samples: use of an algorithm combining microbiologic, serologic, and sequential multiplex PCR techniques.

Authors:  Karen Miernyk; Carolynn Debyle; Marcella Harker-Jones; Kimberlee Boyd Hummel; Thomas Hennessy; Jay Wenger; Karen Rudolph
Journal:  J Clin Microbiol       Date:  2011-07-20       Impact factor: 5.948

8.  Update of pneumococcal PCR serotyping assay for detection of a commonly occurring type 19F wzy variant in Brazil.

Authors:  Ana Paula de O Menezes; Joice Neves Reis; Yves Mauro Ternes; Ana Lúcia Andrade; Fabiana C Pimenta; Maria da Gloria Carvalho; Bernard Beall
Journal:  J Clin Microbiol       Date:  2013-05-08       Impact factor: 5.948

9.  Sentinel versus population-based surveillance of pneumococcal conjugate vaccine effectiveness.

Authors:  Lee M Hampton; Elizabeth R Zell; Stephanie Schrag; Adam L Cohen
Journal:  Bull World Health Organ       Date:  2012-05-11       Impact factor: 9.408

Review 10.  Pneumococcal Capsules and Their Types: Past, Present, and Future.

Authors:  K Aaron Geno; Gwendolyn L Gilbert; Joon Young Song; Ian C Skovsted; Keith P Klugman; Christopher Jones; Helle B Konradsen; Moon H Nahm
Journal:  Clin Microbiol Rev       Date:  2015-07       Impact factor: 26.132
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.