Development of Complete Homozygotes of Tobacco.

True homnozygotes of Nicotiana tabacum L., herein referred to as Ky Iso lines, are being developed from haploids. A technique is described whereby seedlings resulting from fertilization are eliminated, leaving only suspect haploids. After confirmation, haploids are treated to double the chromosomes and these result in completely homozygous plants. One complete homnozygote, Ky Iso 1 Ky 16, has been developed and shows extreme uniformity in all characters studied. The Ky Iso lines will be useful in genetic stuidies that require no plant-to-plant genotypic variability. The Ky Iso lines will be made available upon request as they are developed.