| Literature DB >> 17720863 |
H Y Li1, C-K Park, S J Jung, S-Y Choi, S J Lee, K Park, J S Kim, S B Oh.
Abstract
UNLABELLED: Eugenol, a natural capsaicin congener, is widely used in dentistry. Eugenol inhibits voltage-activated Na(+) and Ca(2+) channels in a transient receptor potential vanilloid 1 (TRPV1)-independent manner. We hypothesized that eugenol also inhibits voltage-gated K(+) currents, and investigated this in rat trigeminal ganglion neurons and in a heterologous system using whole-cell patch clamping. Eugenol inhibited voltage-gated K(+) currents, and the inhibitory effects of eugenol were observed in both capsaicin-sensitive and capsaicin-insensitive neurons. Pre-treatment with capsazepine, a well-known antagonist of TRPV1, failed to block the inhibitory effects of eugenol on K(+) currents, suggesting no involvement of TRPV1. Eugenol inhibited human Kv1.5 currents stably expressed in Ltk(-) cells, where TRPV1 is not endogenously expressed. We conclude that eugenol inhibits voltage-gated K(+) currents in a TRPV1-independent manner. The inhibition of voltage-gated K(+) currents is likely to contribute to the irritable action of eugenol. ABBREVIATIONS: human Kv1.5 channel, hKv1.5; transient receptor potential vanilloid 1, TRPV1.Entities:
Mesh：
Substances：
Year: 2007 PMID： 17720863 DOI： 10.1177/154405910708600918
Source DB: PubMed Journal: J Dent Res ISSN： 0022-0345 Impact factor: 6.116