| Literature DB >> 17720554 |
Noriyoshi Mizuno1, Hideki Shiba, Wan-peng Xu, Takafumi Inui, Tsuyoshi Fujita, Mikihito Kajiya, Katsuhiro Takeda, Naohiko Hasegawa, Hiroyuki Kawaguchi, Hidemi Kurihara.
Abstract
Neurotrophins (NTs) are expressed during tooth development. However, little is known about a role of NTs in differentiation of pulp cells into mineralizing cells. In this study, mRNA expressions of hard tissue-related proteins, calcification and proliferation are examined in cultures of human pulp (HP) cells. Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin (NT)-3 and NT-4/5 increased the mRNA levels of dentin sialophsphoprotein, alkaline phosphatase, osteopontin, type I collagen and bone morphogenetic protein-2 and mineral deposition in cultures of HP cells. The increased levels and manners varied, depending on the concentrations of NTs and hard-tissue related protein tested. On the other hand, only NGF significantly stimulated DNA synthesis in cultures of HP cells. These findings suggest that NTs characteristically regulate hard-tissue related protein expression, calcification and proliferation in pulp cells. NTs may accelerate pulp cell differentiation.Entities:
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Year: 2007 PMID： 17720554 DOI： 10.1016/j.cellbi.2007.06.012
Source DB: PubMed Journal: Cell Biol Int ISSN： 1065-6995 Impact factor: 3.612