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Literature DB >> 17720480

In vitro and in vivo assays for the activity of Drosha complex.

Abstract

MicroRNA (miRNA) genes are transcribed into long primary transcripts (pri-miRNAs) that get processed into mature miRNAs of about 22 nt in length by two different ribonuclease (RNase) III enzymes, Drosha and Dicer. Various experimental protocols have been developed and modified for genetic and biochemical analyses for microRNA processing. Here we describe the methods for the analysis of pri-miRNA processing that is mediated by Drosha and its cofactor, DiGeorge Syndrome Critical Region Gene 8 (DGCR8).

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Year: 2007 PMID： 17720480 DOI： 10.1016/S0076-6879(07)27005-3

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

26 in total

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3. ADAR1 is required for differentiation and neural induction by regulating microRNA processing in a catalytically independent manner.

Authors: Tian Chen; Jian-Feng Xiang; Shanshan Zhu; Siye Chen; Qing-Fei Yin; Xiao-Ou Zhang; Jun Zhang; Hua Feng; Rui Dong; Xue-Jun Li; Li Yang; Ling-Ling Chen
Journal: Cell Res Date: 2015-02-24 Impact factor: 25.617

4. Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing.

Authors: Vincent C Auyeung; Igor Ulitsky; Sean E McGeary; David P Bartel
Journal: Cell Date: 2013-02-14 Impact factor: 41.582

5. Nucleolin protein interacts with microprocessor complex to affect biogenesis of microRNAs 15a and 16.

Authors: Brian F Pickering; Dihua Yu; Michael W Van Dyke
Journal: J Biol Chem Date: 2011-11-02 Impact factor: 5.157

6. Musashi1 cooperates in abnormal cell lineage protein 28 (Lin28)-mediated let-7 family microRNA biogenesis in early neural differentiation.

Authors: Hironori Kawahara; Yohei Okada; Takao Imai; Akio Iwanami; Paul S Mischel; Hideyuki Okano
Journal: J Biol Chem Date: 2011-03-04 Impact factor: 5.157

In vitro and in vivo assays for the activity of Drosha complex.

1. Cell-cell contact globally activates microRNA biogenesis.

2. Kaposi's sarcoma-associated herpesvirus microRNA single-nucleotide polymorphisms identified in clinical samples can affect microRNA processing, level of expression, and silencing activity.

3. ADAR1 is required for differentiation and neural induction by regulating microRNA processing in a catalytically independent manner.

4. Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing.

5. Nucleolin protein interacts with microprocessor complex to affect biogenesis of microRNAs 15a and 16.

6. Musashi1 cooperates in abnormal cell lineage protein 28 (Lin28)-mediated let-7 family microRNA biogenesis in early neural differentiation.

7. Transcriptional profiling reveals that C5a alters microRNA in brain endothelial cells.

8. The core microprocessor component DiGeorge syndrome critical region 8 (DGCR8) is a nonspecific RNA-binding protein.

9. Kaposi's sarcoma-associated herpesvirus encodes a mimic of cellular miR-23.

10. The Menu of Features that Define Primary MicroRNAs and Enable De Novo Design of MicroRNA Genes.