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Literature DB >> 17719814

A method for C(alpha) direct-detection in protonless NMR.

Wolfgang Bermel¹, Ivano Bertini, Isabella C Felli, Manolis Matzapetakis, Roberta Pierattelli, Elizabeth C Theil, Paola Turano.

Abstract

Attempts are made to efficiently decouple (13)C nuclei without significant loss of coherence during the application of the decoupling package. Such attempts are based on the S(3)E spin-state selection method. A newly developed double S(3)E (DS(3)E) is particularly efficient for C(alpha) detection for proteins as large as 480 kDa.

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Year: 2007 PMID： 17719814 DOI： 10.1016/j.jmr.2007.07.004

Source DB: PubMed Journal: J Magn Reson ISSN： 1090-7807 Impact factor: 2.229

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Cited

18 in total

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3. Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.

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4. Alternate 13C-12C labeling for complete mainchain resonance assignments using C alpha direct-detection with applicability toward fast relaxing protein systems.

Authors: Koh Takeuchi; Zhen-Yu J Sun; Gerhard Wagner
Journal: J Am Chem Soc Date: 2008-12-24 Impact factor: 15.419

A method for C(alpha) direct-detection in protonless NMR.

1. Structural and mechanistic implications of metal binding in the small heat-shock protein αB-crystallin.

2. High resolution methyl selective ¹³C-NMR of proteins in solution and solid state.

3. Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.

4. Alternate 13C-12C labeling for complete mainchain resonance assignments using C alpha direct-detection with applicability toward fast relaxing protein systems.

5. SedNMR: a web tool for optimizing sedimentation of macromolecular solutes for SSNMR.

6. Solid-state NMR of proteins sedimented by ultracentrifugation.

7. Improving the chemical shift dispersion of multidimensional NMR spectra of intrinsically disordered proteins.

8. On the use of ultracentrifugal devices for sedimented solute NMR.

9. Coordinating subdomains of ferritin protein cages with catalysis and biomineralization viewed from the C4 cage axes.

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