| Literature DB >> 17719584 |
Kyoung Sun Park1, Ha Young Lee, Sun Young Lee, Mi-Kyoung Kim, Sang Doo Kim, Jung Mo Kim, Jeanho Yun, Dong-Soon Im, Yoe-Sik Bae.
Abstract
We investigated whether lysophosphatidylethanolamine (LPE) modulates cellular signaling in different cell types. SK-OV3 ovarian cancer cells and OVCAR-3 ovarian cancer cells were responsive to LPE. LPE-stimulated intracellular calcium concentration ([Ca(2+)](i)) increase was inhibited by U-73122, suggesting that LPE stimulates calcium signaling via phospholipase C activation. Moreover, pertussis toxin (PTX) almost completely inhibited [Ca(2+)](i) increase by LPE, indicating the involvement of PTX-sensitive G-proteins. Furthermore, we found that LPE stimulated chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells. We examined the role of lysophosphatidic acid receptors on LPE-stimulated cellular responses using HepG2 cells transfected with different LPA receptors, and found that LPE failed to stimulate nuclear factor kappa B-driven luciferase. We suggest that LPE stimulates a membrane bound receptor, different from well known LPA receptors, resulting in chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells.Entities:
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Year: 2007 PMID： 17719584 DOI： 10.1016/j.febslet.2007.08.014
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124