AIMS: Isolation and antimicrobial evaluation of aquatic bacterial strains from two cenotes. METHODS AND RESULTS: A total of 258 bacterial strains were isolated from the water and sediment of two cenotes in the Yucatan peninsula, all of which were screened against six pathogenic micro-organisms. Antimicrobial activity was detected in 46 of the isolated strains against at least one of the target strains tested. Antimicrobially active isolates were identified as: Aeromonas, Bacillus, Burkholderia, Photobacterium, Pseudomonas, Serratia, Shewanella, Stenotrophomonas genera, and 13 remained unidentified. All antimicrobially active strains were able to grow in salt medium at a concentration of 75 g l(-1), thus classifying as moderately halotolerant bacteria. Most of the antimicrobially active strains exhibited a broad action spectrum, where 61% was because of uncharacterized antimicrobial substances, 25% because of bacteriocins and 13% because of siderophores. Ten strains were able to biosynthesize biosurfactant metabolites. CONCLUSIONS: Native bacteria from the Yucatan peninsula showed an interesting antimicrobial activity, diverse mode of action and moderate halotolerance to salt. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report on bacterial isolates from cenotes of the Yucatan peninsula and their antimicrobial characterization, with great potential for future biotechnological applications.
AIMS: Isolation and antimicrobial evaluation of aquatic bacterial strains from two cenotes. METHODS AND RESULTS: A total of 258 bacterial strains were isolated from the water and sediment of two cenotes in the Yucatan peninsula, all of which were screened against six pathogenic micro-organisms. Antimicrobial activity was detected in 46 of the isolated strains against at least one of the target strains tested. Antimicrobially active isolates were identified as: Aeromonas, Bacillus, Burkholderia, Photobacterium, Pseudomonas, Serratia, Shewanella, Stenotrophomonas genera, and 13 remained unidentified. All antimicrobially active strains were able to grow in salt medium at a concentration of 75 g l(-1), thus classifying as moderately halotolerant bacteria. Most of the antimicrobially active strains exhibited a broad action spectrum, where 61% was because of uncharacterized antimicrobial substances, 25% because of bacteriocins and 13% because of siderophores. Ten strains were able to biosynthesize biosurfactant metabolites. CONCLUSIONS: Native bacteria from the Yucatan peninsula showed an interesting antimicrobial activity, diverse mode of action and moderate halotolerance to salt. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report on bacterial isolates from cenotes of the Yucatan peninsula and their antimicrobial characterization, with great potential for future biotechnological applications.
Authors: Melanie J Beazley; Robert J Martinez; Suja Rajan; Jessica Powell; Yvette M Piceno; Lauren M Tom; Gary L Andersen; Terry C Hazen; Joy D Van Nostrand; Jizhong Zhou; Behzad Mortazavi; Patricia A Sobecky Journal: PLoS One Date: 2012-07-18 Impact factor: 3.240
Authors: Carlos A Fajardo-Hernández; Firoz Shah Tuglak Khan; Laura Flores-Bocanegra; Alejandra Prieto-Davó; Baojie Wan; Rui Ma; Mallique Qader; Rodrigo Villanueva-Silva; Anahí Martínez-Cárdenas; Marian A López-Lobato; Shabnam Hematian; Scott G Franzblau; Huzefa A Raja; Rodolfo García-Contreras; Mario Figueroa Journal: ACS Omega Date: 2022-03-28
Authors: Pedro Seoane; Silvana T Tapia-Paniagua; Rocío Bautista; Elena Alcaide; Consuelo Esteve; Eduardo Martínez-Manzanares; M Carmen Balebona; M Gonzalo Claros; Miguel A Moriñigo Journal: PeerJ Date: 2019-02-28 Impact factor: 2.984
Authors: Félix Maldonado Desena; Navila De la Cruz Ceferino; Sergio Gómez Cornelio; Carina Alvarez Villagomez; José Luis Herrera Candelario; Susana De la Rosa García Journal: Microorganisms Date: 2022-06-21