OBJECTIVE: The purpose of this study was to determine whether a high-salt diet modulates physiological angiogenesis in skeletal muscle by altering angiotensin II (ANGII) and vascular endothelial growth factor (VEGF) levels. METHODS: Sprague-Dawley rats were placed on a control diet (0.4% NaCl by weight) or high-salt diet (4.0% NaCl) prior to treatment with the vasodilator prazosin in the drinking water. In addition, a group of animals fed high salt were infused intravenously with ANGII at a low dose to prevent ANGII suppression by high salt, and a group of rats fed control diet were treated with the angiotensin II type I (AT(1)) receptor blocker losartan and prazosin. RESULTS: Prazosin induced significant angiogenesis in the tibialis anterior muscle after 1 week of treatment. High-salt-fed rats demonstrated a complete inhibition of this angiogenic response. Maintenance of ANGII levels restored prazosin-induced angiogenesis in animals fed a high-salt diet. In addition, losartan treatment blocked prazosin-induced angiogenesis in animals on a control diet. Western blot analysis indicated that prazosin-induced angiogenesis was independent of changes in muscle levels of VEGF. CONCLUSIONS: This study demonstrates an inhibitory effect of high salt intake on prazosin-induced angiogenesis. Further, these results indicate that ANGII acting through the AT(1) receptor is a critical pathway in this model of angiogenesis.
OBJECTIVE: The purpose of this study was to determine whether a high-salt diet modulates physiological angiogenesis in skeletal muscle by altering angiotensin II (ANGII) and vascular endothelial growth factor (VEGF) levels. METHODS:Sprague-Dawley rats were placed on a control diet (0.4% NaCl by weight) or high-salt diet (4.0% NaCl) prior to treatment with the vasodilator prazosin in the drinking water. In addition, a group of animals fed high salt were infused intravenously with ANGII at a low dose to prevent ANGII suppression by high salt, and a group of rats fed control diet were treated with the angiotensin II type I (AT(1)) receptor blocker losartan and prazosin. RESULTS:Prazosin induced significant angiogenesis in the tibialis anterior muscle after 1 week of treatment. High-salt-fed rats demonstrated a complete inhibition of this angiogenic response. Maintenance of ANGII levels restored prazosin-induced angiogenesis in animals fed a high-salt diet. In addition, losartan treatment blocked prazosin-induced angiogenesis in animals on a control diet. Western blot analysis indicated that prazosin-induced angiogenesis was independent of changes in muscle levels of VEGF. CONCLUSIONS: This study demonstrates an inhibitory effect of high salt intake on prazosin-induced angiogenesis. Further, these results indicate that ANGII acting through the AT(1) receptor is a critical pathway in this model of angiogenesis.
Authors: Radia Tamarat; Jean-Sébastien Silvestre; Nathalie Kubis; Joelle Benessiano; Micheline Duriez; Marc deGasparo; Daniel Henrion; Bernard I Levy Journal: Hypertension Date: 2002-03-01 Impact factor: 10.190
Authors: Oliver Baum; Luis Da Silva-Azevedo; Gregor Willerding; Achim Wöckel; Gerit Planitzer; Reinhart Gossrau; Axel R Pries; Andreas Zakrzewicz Journal: Am J Physiol Heart Circ Physiol Date: 2004-07-01 Impact factor: 4.733
Authors: Sandra L Amaral; Kristopher G Maier; Daniela N Schippers; Richard J Roman; Andrew S Greene Journal: Am J Physiol Heart Circ Physiol Date: 2003-01-09 Impact factor: 4.733
Authors: Timothy J Stodola; Micheline M de Resende; Allison B Sarkis; Daniela N Didier; Howard J Jacob; Norbert Huebner; Oliver Hummel; Kathrin Saar; Carol Moreno; Andrew S Greene Journal: Physiol Genomics Date: 2011-04-26 Impact factor: 3.107
Authors: Sander van Ginkel; Arnold de Haan; Jorn Woerdeman; Luc Vanhees; Erik Serné; Jos de Koning; Martin Flück Journal: Appl Transl Genom Date: 2015-03-27