B Shivananda Nayak1, S Sivachandra Raju, A V Chalapathi Rao. 1. Department of Pre-Clinical Sciences, Biochemistry Unit, Faculty of Medical Sciences,The University of the West Indies, St. Augustine,Trinidad. shiv25@gmail.com
Abstract
OBJECTIVE: To evaluate the wound healing activity of M. recutita (chamomile) extract in rats. METHOD: Wound healing activity was determined using excision, incision and dead space wound models. The animals were divided into two groups of six for each model: animals in the test group were treated with the aqueous extract of M. recutita (120mg/kg/day), which was mixed in their drinking water. Animals in the control group were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialisation, wound-breaking strength, granulation tissue weight and hydoxyproline content. Antimicrobial activity of the extract against various microorganisms was assessed. RESULTS: On day 15 animals in the test group exhibited a greater reduction in the wound area when compared with the controls (61 % versus 48%), faster epithelialisation and a significantly higher wound-breaking strength (p<0.002). In addition, wet and dry granulation tissue weight and hydroxyproline content were significantly higher. CONCLUSION: The increased rate of wound contraction, together with the increased wound-breaking strength, hydroxyproline content and histological observations, support the use of M. recutita in wound management. However, this needs to be studied further before it can be considered for clinical use.
OBJECTIVE: To evaluate the wound healing activity of M. recutita (chamomile) extract in rats. METHOD: Wound healing activity was determined using excision, incision and dead space wound models. The animals were divided into two groups of six for each model: animals in the test group were treated with the aqueous extract of M. recutita (120mg/kg/day), which was mixed in their drinking water. Animals in the control group were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialisation, wound-breaking strength, granulation tissue weight and hydoxyproline content. Antimicrobial activity of the extract against various microorganisms was assessed. RESULTS: On day 15 animals in the test group exhibited a greater reduction in the wound area when compared with the controls (61 % versus 48%), faster epithelialisation and a significantly higher wound-breaking strength (p<0.002). In addition, wet and dry granulation tissue weight and hydroxyproline content were significantly higher. CONCLUSION: The increased rate of wound contraction, together with the increased wound-breaking strength, hydroxyproline content and histological observations, support the use of M. recutita in wound management. However, this needs to be studied further before it can be considered for clinical use.
Authors: Hossein Salehi; Mohammad Mehrasa; Bijan Nasri-Nasrabadi; Mohsen Doostmohammadi; Reihaneh Seyedebrahimi; Navid Davari; Mohammad Rafienia; Mehdi E Hosseinabadi; Maria Agheb; Mansour Siavash Journal: J Res Med Sci Date: 2017-09-26 Impact factor: 1.852