BACKGROUND/AIMS: Tumor may induce local immunosuppression and make the tumor-infiltrating lymphocytes (TILs) functionally inhibited and lose the antitumor effects. Therefore, we did the phenotypic analysis of TILs in hepatocellular carcinoma (HCC) tissues. METHODOLOGY: Lymphocytes were isolated from paired HCC tissues (TILs) and the corresponding non-tumor liver tissues (non-tumor-liver infiltrating lymphocytes, NILs) of 28 patients and were subjected to flow cytometric analysis. RESULTS: Compared with the non-tumor portion, HCC tissues had less intensity of lymphocyte infiltration. TILs had higher CD3+/CD56+ ratio than NILs. Around 70%-90% NILs or TILs expressed the antigen-experienced or memory phenotypes. Around 60%-70% CD4+ or CD8+ NILs and TILs expressed the activation markers CD69 and HLA-DR. However, we found that CD25 is under-expressed in both the CD8+ NILs and TILs. In addition, more percentage of CD4+ CD25+ T cells was detected in the TILs than in the NILs. CONCLUSIONS: HCC tissues had less lymphocytes infiltration. Most of infiltrating lymphocytes from the HCC tissues and the corresponding non-tumor liver tissues were activated and expressed antigen-experienced phenotypes. However, the CD25 was underexpressed in the CD8+ TILs and the CD4+ CD25+ T cells were increased in the TILs. These factors might impair the antitumor immunity in HCCs.
BACKGROUND/AIMS: Tumor may induce local immunosuppression and make the tumor-infiltrating lymphocytes (TILs) functionally inhibited and lose the antitumor effects. Therefore, we did the phenotypic analysis of TILs in hepatocellular carcinoma (HCC) tissues. METHODOLOGY: Lymphocytes were isolated from paired HCC tissues (TILs) and the corresponding non-tumor liver tissues (non-tumor-liver infiltrating lymphocytes, NILs) of 28 patients and were subjected to flow cytometric analysis. RESULTS: Compared with the non-tumor portion, HCC tissues had less intensity of lymphocyte infiltration. TILs had higher CD3+/CD56+ ratio than NILs. Around 70%-90% NILs or TILs expressed the antigen-experienced or memory phenotypes. Around 60%-70% CD4+ or CD8+ NILs and TILs expressed the activation markers CD69 and HLA-DR. However, we found that CD25 is under-expressed in both the CD8+ NILs and TILs. In addition, more percentage of CD4+ CD25+ T cells was detected in the TILs than in the NILs. CONCLUSIONS: HCC tissues had less lymphocytes infiltration. Most of infiltrating lymphocytes from the HCC tissues and the corresponding non-tumor liver tissues were activated and expressed antigen-experienced phenotypes. However, the CD25 was underexpressed in the CD8+ TILs and the CD4+ CD25+ T cells were increased in the TILs. These factors might impair the antitumor immunity in HCCs.
Authors: Nicolas Jacquelot; María Paula Roberti; David P Enot; Sylvie Rusakiewicz; Michaela Semeraro; Sarah Jégou; Camila Flores; Lieping Chen; Byoung S Kwon; Christophe Borg; Benjamin Weide; François Aubin; Stéphane Dalle; Holbrook Kohrt; Maha Ayyoub; Guido Kroemer; Aurélien Marabelle; Andréa Cavalcanti; Alexander Eggermont; Laurence Zitvogel Journal: J Invest Dermatol Date: 2016-01-29 Impact factor: 8.551
Authors: Cesar Miguel Momesso Dos Santos; Vinicius Leonardo Sousa Diniz; André Luis Lacerda Bachi; Laiane Cristina Dos Santos de Oliveira; Tamara Ghazal; Maria Elizabeth Pereira Passos; Heloisa Helena de Oliveira; Gilson Murata; Laureane Nunes Masi; Amanda Roque Martins; Adriana Cristina Levada-Pires; Rui Curi; Sandro Massao Hirabara; Donald F Sellitti; Tania Cristina Pithon-Curi; Renata Gorjão Journal: Nutr Metab (Lond) Date: 2019-09-12 Impact factor: 4.169