| Literature DB >> 17707815 |
Yuan Gu1, Jie Li, Xinping Zhu, Jing Yang, Qiang Li, Zhiying Liu, Shun Yu, Yaohua Li.
Abstract
Trichinellosis is a global zoonosis mainly caused by Trichinella spiralis. We have previously reported that a novel Ts87 gene from the cDNA library of adult T. spiralis was cloned and expressed in a prokaryotic expression system. Vaccination with recombinant Ts87 protein (rTs87) induced a muscle larvae burden reduction in BALB/c mice by 29% in response to T. spiralis infection. In the present study, we screened a random phage-displayed peptide library using monoclonal antibody 5A3 which recognized Ts87 protein. Four positive phage clones were selected to subcutaneously immunize BALB/c mice without adjuvant. Two phage clones could effectively stimulate specific antibodies against rTs87. Mice vaccinated with these two combined phage clones showed a 28.7% worm burden reduction as compared to the control group. Therefore, the identified phage clones displayed peptides representing specific epitopes of Ts87 protein and could be considered as potential vaccine candidates for T. spiralis.Entities:
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Year: 2007 PMID： 17707815 DOI： 10.1016/j.exppara.2007.06.014
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011