| Literature DB >> 17706596 |
Young Soo Yoon1, Jung Ha Choo1, Taekyung Yoo1, Keunsoo Kang1, Jae Hoon Chung2.
Abstract
RhoB, a member of the Rho family of small GTPases, regulates the organization of the actin cytoskeleton, malignant transformation, and genotoxic stress-induced signaling. In order to characterize epigenetic regulation of RhoB transcription during aging, the mRNA levels of RhoB were investigated using various mouse tissues of different ages. Bisulfite sequencing revealed that the CpG dinucleotide methylation patterns of the RhoB promoter region were not altered in skeletal muscle and lung during aging. ChIP analysis showed that levels of histone H3 and H4 acetylation were reduced in a tissue-specific manner during aging due to direct HDAC1 binding. Histone H3 lysine 9 trimethylation level and deposition of HP1beta increased in RhoB promoter during aging, whereas histone H3 lysine 4 dimethylation level gradually decreased. It was concluded that mouse RhoB transcription is epigenetically regulated in a tissue-specific manner during aging by histone modification, but not by CpG methylation.Entities:
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Year: 2007 PMID： 17706596 DOI： 10.1016/j.bbrc.2007.08.002
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575