| Literature DB >> 17706421 |
S-W Chang1, J-F Shaw, K-H Yang, S-F Chang, C-J Shieh.
Abstract
Poly(gamma-glutamic acid) (gamma-PGA) is a material of polymer. Immobilization of Candida rugosa lipase (Lipase AY-30) by covalent binding on gamma-PGA led to a markedly improved performance of the enzyme. Response surface methodology (RSM) and 3-level-3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (2-6h), immobilization temperature (0-26 degrees C), and enzyme/support ratio (0.1-0.5, w/w). Based on the analysis of ridge max, the optimum immobilization conditions were as follows: immobilization time 2.3h, immobilization temperature 13.3 degrees C, and enzyme/support ratio 0.41 (w/w); the highest lipase activity obtained was 1196 U/mg-protein.Entities:
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Year: 2007 PMID： 17706421 DOI： 10.1016/j.biortech.2007.06.020
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642