OBJECTIVE: To report on the development of human parthenogenetic blastocysts and an in vitro attachment that was generated from noninseminated cryopreserved human oocytes for the first time. DESIGN: Prospective study. SETTING: Department of reproductive medicine in a medical institute in Buenos Aires, Argentina. PATIENT(S): Five healthy fertile donors. INTERVENTION(S): Artificial activation of noninseminated cryopreserved human oocytes after thawing, parthenote culture, and their in vitro attachment. MAIN OUTCOME MEASURE(S): Survival rate, activation rate, cleavage rate, and blastocyst formation. RESULT(S): Thirty-six of 38 cryopreserved noninseminated oocytes survived after thawing (survival rate, 94.7%). Thirty-one of 36 oocytes showed one pronucleus (activation rate, 86.1%). Thirty of 31 cleaved (cleavage rate, 96.8%). Five of 30 showed cavitation (blastocyst rate, 16.7%). CONCLUSION(S): Noninseminated cryopreserved human oocytes showed a high survival rate after thawing. They responded very satisfactorily to artificial activation, which was followed by a high rate of parthenogenetic embryos, which can develop into blastocysts. In the future, these could be a new source for development of human parthenogenetic stem cells.
OBJECTIVE: To report on the development of human parthenogenetic blastocysts and an in vitro attachment that was generated from noninseminated cryopreserved human oocytes for the first time. DESIGN: Prospective study. SETTING: Department of reproductive medicine in a medical institute in Buenos Aires, Argentina. PATIENT(S): Five healthy fertile donors. INTERVENTION(S): Artificial activation of noninseminated cryopreserved human oocytes after thawing, parthenote culture, and their in vitro attachment. MAIN OUTCOME MEASURE(S): Survival rate, activation rate, cleavage rate, and blastocyst formation. RESULT(S): Thirty-six of 38 cryopreserved noninseminated oocytes survived after thawing (survival rate, 94.7%). Thirty-one of 36 oocytes showed one pronucleus (activation rate, 86.1%). Thirty of 31 cleaved (cleavage rate, 96.8%). Five of 30 showed cavitation (blastocyst rate, 16.7%). CONCLUSION(S): Noninseminated cryopreserved human oocytes showed a high survival rate after thawing. They responded very satisfactorily to artificial activation, which was followed by a high rate of parthenogenetic embryos, which can develop into blastocysts. In the future, these could be a new source for development of human parthenogenetic stem cells.
Authors: Christian S Ottolini; Antonio Capalbo; Louise Newnham; Danilo Cimadomo; Senthilkumar A Natesan; Eva R Hoffmann; Filippo M Ubaldi; Laura Rienzi; Alan H Handyside Journal: Nat Protoc Date: 2016-06-16 Impact factor: 13.491
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