Tingting Ding1, Jiao Sun, Ping Zhang. 1. Ninth People's Hospital School of Medicine, Shanghai Jiao tong University/Shanghai Biomaterials Research & Testing Center, Shanghai 200023, China.
Abstract
OBJECTIVE: To examine biomaterial biocompatibility and improve current methods of immunological evaluation TNF-alpha and IL1-beta were used as indicators at mRNA levels. METHODS: Rat peritoneal macrophages were stimulated with different biomaterials and expression of TNF-alpha and IL1-beta was measured by RT-PCR and compared within different groups. RESULTS: Macrophages that were stimulated with PTFE produced significantly more TNF-alpha and IL1-beta than unstimulated macrophages (p < 0.001). The PLGA, NPG, beta-TCP, and CPC groups also induced moderate TNF-alpha and IL1-beta expression (p < 0.01). CONCLUSIONS: TNF-alpha and IL1-beta are sensitive indicators of immune stimulation that can help to monitor the levels of cellular activation induced by different biomaterials. Our findings showed that beta-TCP and CPC had a good biocompability, and CPC was the most biocompatible of all the biomaterials tested. While PTFE and NPG still had side effect because of producing pro-inflammatory cytokines in vitro once implanted.
OBJECTIVE: To examine biomaterial biocompatibility and improve current methods of immunological evaluation TNF-alpha and IL1-beta were used as indicators at mRNA levels. METHODS:Rat peritoneal macrophages were stimulated with different biomaterials and expression of TNF-alpha and IL1-beta was measured by RT-PCR and compared within different groups. RESULTS: Macrophages that were stimulated with PTFE produced significantly more TNF-alpha and IL1-beta than unstimulated macrophages (p < 0.001). The PLGA, NPG, beta-TCP, and CPC groups also induced moderate TNF-alpha and IL1-beta expression (p < 0.01). CONCLUSIONS:TNF-alpha and IL1-beta are sensitive indicators of immune stimulation that can help to monitor the levels of cellular activation induced by different biomaterials. Our findings showed that beta-TCP and CPC had a good biocompability, and CPC was the most biocompatible of all the biomaterials tested. While PTFE and NPG still had side effect because of producing pro-inflammatory cytokines in vitro once implanted.
Authors: G Basta; P Sarchielli; G Luca; L Racanicchi; C Nastruzzi; L Guido; F Mancuso; G Macchiarulo; G Calabrese; P Brunetti; R Calafiore Journal: Transpl Immunol Date: 2004-12 Impact factor: 1.708
Authors: Jan Hoffmann; Perikles Simon; Anja K Zimmermann; Michael Lemancyk; Thomas Walter; Martin Beyer; Hans-Martin Hoffmeister; Gerhard Ziemer; Hans P Wendel Journal: Biomaterials Date: 2005-09 Impact factor: 12.479
Authors: Gian Gastone Neri Serneri; Maria Boddi; Pietro Amedeo Modesti; Ilaria Cecioni; Mirella Coppo; Maria Letizia Papa; Thomas Toscano; Antonio Marullo; Mario Chiavarelli Journal: Circ Res Date: 2003-05-29 Impact factor: 17.367
Authors: M Hummel; S Czerlinski; N Friedel; C Liebenthal; D Hasper; R von Baehr; R Hetzer; H D Volk Journal: Crit Care Med Date: 1994-03 Impact factor: 7.598
Authors: Ryan Gene Soderquist; Evan M Sloane; Lisa C Loram; Jacqueline A Harrison; Ellen C Dengler; Scott M Johnson; Luke D Amer; Courtney S Young; Makenzie T Lewis; Stephen Poole; Matthew G Frank; Linda R Watkins; Erin D Milligan; Melissa J Mahoney Journal: Pharm Res Date: 2010-03-12 Impact factor: 4.200
Authors: Jay C Sy; Gokulakrishnan Seshadri; Stephen C Yang; Milton Brown; Teresa Oh; Sergey Dikalov; Niren Murthy; Michael E Davis Journal: Nat Mater Date: 2008-10-19 Impact factor: 43.841
Authors: Sahil Shah; Saeed Daneshmandi; Kevin R Hughes; Shuangjin Yu; Angela M Bedoya; Lonnie D Shea; Xunrong Luo Journal: Biomaterials Date: 2019-05-01 Impact factor: 12.479