Literature DB >> 17703216

Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays.

Mayka Sanchez1, Bruno Galy, Matthias W Hentze, Martina U Muckenthaler.   

Abstract

RNA-binding proteins (RBPs) frequently regulate the post-transcriptional fate of target mRNAs. To identify novel target mRNAs of RBPs, we incubate total RNA with recombinant RBP and immunoselect the messenger-ribonucleoproteins using a specific anti-RBP antibody. The mRNA composition of the supernatant and/or immunoprecipitated fraction is analyzed using dual-color microarrays in comparison with control reaction. From start to finish, the protocol takes approximately 6 d.

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Year:  2007        PMID： 17703216     DOI： 10.1038/nprot.2007.293

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  6 in total

1.  Dissecting the expression dynamics of RNA-binding proteins in posttranscriptional regulatory networks.

Authors:  Nitish Mittal; Nilanjan Roy; M Madan Babu; Sarath Chandra Janga
Journal:  Proc Natl Acad Sci U S A       Date:  2009-11-16       Impact factor: 11.205

2.  RNase L controls terminal adipocyte differentiation, lipids storage and insulin sensitivity via CHOP10 mRNA regulation.

Authors:  O Fabre; T Salehzada; K Lambert; Y Boo Seok; A Zhou; J Mercier; C Bisbal
Journal:  Cell Death Differ       Date:  2012-03-23       Impact factor: 15.828

3.  Siderophore-mediated iron trafficking in humans is regulated by iron.

Authors:  Zhuoming Liu; Robert Lanford; Sebastian Mueller; Glenn S Gerhard; Sara Luscieti; Mayka Sanchez; L Devireddy
Journal:  J Mol Med (Berl)       Date:  2012-04-15       Impact factor: 4.599

4.  An iron responsive element-like stem-loop regulates alpha-hemoglobin-stabilizing protein mRNA.

Authors:  Camila O dos Santos; Louis C Dore; Eric Valentine; Suresh G Shelat; Ross C Hardison; Manik Ghosh; Wei Wang; Richard S Eisenstein; Fernando F Costa; Mitchell J Weiss
Journal:  J Biol Chem       Date:  2008-08-02       Impact factor: 5.157

5.  Arabidopsis Pumilio protein APUM5 suppresses Cucumber mosaic virus infection via direct binding of viral RNAs.

Authors:  Sung Un Huh; Min Jung Kim; Kyung-Hee Paek
Journal:  Proc Natl Acad Sci U S A       Date:  2012-12-26       Impact factor: 11.205

6.  A link between adipogenesis and innate immunity: RNase-L promotes 3T3-L1 adipogenesis by destabilizing Pref-1 mRNA.

Authors:  Yi-Ting Wang; Hou-Hsien Chiang; Ying-Shing Huang; Chia-Lang Hsu; Po-Jen Yang; Hsueh-Fen Juan; Wei-Shiung Yang
Journal:  Cell Death Dis       Date:  2016-11-10       Impact factor: 8.469
  6 in total

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