BACKGROUND: Bone marrow-derived endothelial progenitor cells (EPCs) have been detected in the peripheral blood of patients following thermal injury. EPCs migrate to sites of active neovascularization in response to mediators released after trauma, contributing to wound healing. The aim was to characterize levels and kinetics of EPCs in burned patients, then relate these to key mobilizing factors, vascular endothelial growth factor (VEGF) and the chemokine (C-X-C motif) ligand 12 (CXCL 12), and compare them with those in healthy subjects. METHODS: The study included 19 adult patients with superficial or full-thickness burns and 50 blood donor volunteer controls. EPCs, identified by cell surface markers CD45(dim/-), CD133+, CD144+ and VEGF receptor 2, were quantified by four-colour flow cytometry. Plasma VEGF and CXCL12 were measured using enzyme-linked immunosorbent assay. RESULTS: Burned patients showed a rapid rise in EPC levels within 24 h, a ninefold increase compared with controls, returning to basal levels by 72 h. Body surface area burned correlated strongly with the degree of mobilization. EPC levels correlated significantly with rises in plasma VEGF and CXCL12. CONCLUSION: Thermal injury induced a rapid rise in EPCs that was proportional to the extent of the burn and significantly correlated with levels of angiogenic cytokines. Such cytokines may be used to stimulate EPCs as a future therapeutic target in burned patients. 2007 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.
BACKGROUND: Bone marrow-derived endothelial progenitor cells (EPCs) have been detected in the peripheral blood of patients following thermal injury. EPCs migrate to sites of active neovascularization in response to mediators released after trauma, contributing to wound healing. The aim was to characterize levels and kinetics of EPCs in burned patients, then relate these to key mobilizing factors, vascular endothelial growth factor (VEGF) and the chemokine (C-X-C motif) ligand 12 (CXCL 12), and compare them with those in healthy subjects. METHODS: The study included 19 adult patients with superficial or full-thickness burns and 50 blood donor volunteer controls. EPCs, identified by cell surface markers CD45(dim/-), CD133+, CD144+ and VEGF receptor 2, were quantified by four-colour flow cytometry. Plasma VEGF and CXCL12 were measured using enzyme-linked immunosorbent assay. RESULTS: Burned patients showed a rapid rise in EPC levels within 24 h, a ninefold increase compared with controls, returning to basal levels by 72 h. Body surface area burned correlated strongly with the degree of mobilization. EPC levels correlated significantly with rises in plasma VEGF and CXCL12. CONCLUSION:Thermal injury induced a rapid rise in EPCs that was proportional to the extent of the burn and significantly correlated with levels of angiogenic cytokines. Such cytokines may be used to stimulate EPCs as a future therapeutic target in burned patients. 2007 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.
Authors: Kakali Sarkar; Sergio Rey; Xianjie Zhang; Raul Sebastian; Guy P Marti; Karen Fox-Talbot; Amanda V Cardona; Junkai Du; Yee Sun Tan; Lixin Liu; Frank Lay; Frank J Gonzalez; John W Harmon; Gregg L Semenza Journal: Cardiovasc Res Date: 2011-10-25 Impact factor: 10.787
Authors: Xianjie Zhang; Xiaofei Wei; Lixin Liu; Guy P Marti; Mohammed S Ghanamah; Muhammad J Arshad; Lori Strom; Robert Spence; James Jeng; Stephen Milner; John W Harmon; Gregg L Semenza Journal: Arch Surg Date: 2010-03
Authors: Ibhar Al Mheid; Salim S Hayek; Yi-An Ko; Faysal Akbik; Qunna Li; Nima Ghasemzadeh; Greg S Martin; Qi Long; Muhammad Hammadah; A Maziar Zafari; Viola Vaccarino; Edmund K Waller; Arshed A Quyyumi Journal: Circ Res Date: 2016-07-19 Impact factor: 17.367
Authors: Chrisoula A Toupadakis; Jennifer L Granick; Myrrh Sagy; Alice Wong; Ehssan Ghassemi; Dai-Jung Chung; Dori L Borjesson; Clare E Yellowley Journal: Cytotherapy Date: 2013-07-03 Impact factor: 5.414
Authors: Todd A Baker; Jacqueline Romero; Harold H Bach; Joel A Strom; Richard L Gamelli; Matthias Majetschak Journal: Crit Care Med Date: 2012-08 Impact factor: 7.598
Authors: Doris A Taylor; Emerson C Perin; James T Willerson; Claudia Zierold; Micheline Resende; Marjorie Carlson; Belinda Nestor; Elizabeth Wise; Aaron Orozco; Carl J Pepine; Timothy D Henry; Stephen G Ellis; David X M Zhao; Jay H Traverse; John P Cooke; Robert C Schutt; Aruni Bhatnagar; Maria B Grant; Dejian Lai; Brian H Johnstone; Shelly L Sayre; Lem Moyé; Ray F Ebert; Roberto Bolli; Robert D Simari; Christopher R Cogle Journal: Cell Transplant Date: 2015-11-19 Impact factor: 4.064