Literature DB >> 17699645

Validation of nasopharyngeal sampling and culture techniques for detection of Streptococcus pneumoniae in children in Kenya.

Osman Abdullahi1, Eva Wanjiru, Robert Musyimi, Nina Glass, J Anthony G Scott.   

Abstract

We compared nasopharyngeal swabs against nasal wash cultures for detecting colonizing pneumococci and examined the effect of frozen storage in skim milk-tryptone-glucose-glycerin on culture. Among the 55 children with positive nasal wash cultures, swab cultures were positive for 47 (85%). Of the 96 swabs positive on direct plating, 94 (98%) were positive when recultured after freezing.

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Year:  2007        PMID： 17699645      PMCID： PMC2045369          DOI： 10.1128/JCM.01393-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  3 in total

1.  Spread of Streptococcus pneumoniae in families. I. Carriage rates and distribution of types.

Authors:  J O Hendley; M A Sande; P M Stewart; J M Gwaltney
Journal:  J Infect Dis       Date:  1975-07       Impact factor: 5.226

2.  Report from a WHO working group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae.

Authors:  Katherine L O'Brien; Hanna Nohynek
Journal:  Pediatr Infect Dis J       Date:  2003-02       Impact factor: 2.129

3.  The descriptive epidemiology of Streptococcus pneumoniae and Haemophilus influenzae nasopharyngeal carriage in children and adults in Kilifi district, Kenya.

Authors:  Osman Abdullahi; Joyce Nyiro; Pole Lewa; Mary Slack; J Anthony G Scott
Journal:  Pediatr Infect Dis J       Date:  2008-01       Impact factor: 2.129
  3 in total
  10 in total

1.  Multiple colonization with S. pneumoniae before and after introduction of the seven-valent conjugated pneumococcal polysaccharide vaccine.

Authors:  Silvio D Brugger; Pascal Frey; Suzanne Aebi; Jason Hinds; Kathrin Mühlemann
Journal:  PLoS One       Date:  2010-07-16       Impact factor: 3.240

2.  Detection of Streptococcus pneumoniae strain cocolonization in the nasopharynx.

Authors:  Silvio D Brugger; Lucy J Hathaway; Kathrin Mühlemann
Journal:  J Clin Microbiol       Date:  2009-04-22       Impact factor: 5.948

3.  Rates of acquisition and clearance of pneumococcal serotypes in the nasopharynges of children in Kilifi District, Kenya.

Authors:  Osman Abdullahi; Angela Karani; Caroline C Tigoi; Daisy Mugo; Stella Kungu; Eva Wanjiru; Jane Jomo; Robert Musyimi; Marc Lipsitch; J Anthony G Scott
Journal:  J Infect Dis       Date:  2012-07-24       Impact factor: 5.226

4.  Differential effects of frozen storage on the molecular detection of bacterial taxa that inhabit the nasopharynx.

Authors:  Brenda A Kwambana; Nuredin I Mohammed; David Jeffries; Mike Barer; Richard A Adegbola; Martin Antonio
Journal:  BMC Clin Pathol       Date:  2011-01-24

5.  The prevalence and risk factors for pneumococcal colonization of the nasopharynx among children in Kilifi District, Kenya.

Authors:  Osman Abdullahi; Angela Karani; Caroline C Tigoi; Daisy Mugo; Stella Kungu; Eva Wanjiru; Jane Jomo; Robert Musyimi; Marc Lipsitch; J Anthony G Scott
Journal:  PLoS One       Date:  2012-02-20       Impact factor: 3.240

6.  Human nasal challenge with Streptococcus pneumoniae is immunising in the absence of carriage.

Authors:  Adam K A Wright; Daniela M Ferreira; Jenna F Gritzfeld; Angela D Wright; Kathryn Armitage; Kondwani C Jambo; Emily Bate; Sherouk El Batrawy; Andrea Collins; Stephen B Gordon
Journal:  PLoS Pathog       Date:  2012-04-05       Impact factor: 6.823

7.  Field evaluation of culture plus latex sweep serotyping for detection of multiple pneumococcal serotype colonisation in infants and young children.

Authors:  Paul Turner; Claudia Turner; Auscharee Jankhot; Kawalee Phakaudom; Francois Nosten; David Goldblatt
Journal:  PLoS One       Date:  2013-07-02       Impact factor: 3.240

Review 8.  Pneumococcal carriage in sub-Saharan Africa--a systematic review.

Authors:  Effua Usuf; Christian Bottomley; Richard A Adegbola; Andrew Hall
Journal:  PLoS One       Date:  2014-01-20       Impact factor: 3.240

9.  Multi-serotype pneumococcal nasopharyngeal carriage prevalence in vaccine naïve Nepalese children, assessed using molecular serotyping.

Authors:  Rama Kandasamy; Meeru Gurung; Anushil Thapa; Susan Ndimah; Neelam Adhikari; David R Murdoch; Dominic F Kelly; Denise E Waldron; Katherine A Gould; Stephen Thorson; Shrijana Shrestha; Jason Hinds; Andrew J Pollard
Journal:  PLoS One       Date:  2015-02-02       Impact factor: 3.240

10.  Estimating the contribution of HIV-infected adults to household pneumococcal transmission in South Africa, 2016-2018: A hidden Markov modelling study.

Authors:  Deus Thindwa; Nicole Wolter; Amy Pinsent; Maimuna Carrim; John Ojal; Stefano Tempia; Jocelyn Moyes; Meredith McMorrow; Jackie Kleynhans; Anne von Gottberg; Neil French; Cheryl Cohen; Stefan Flasche
Journal:  PLoS Comput Biol       Date:  2021-12-23       Impact factor: 4.475
  10 in total

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