| Literature DB >> 17697671 |
Suresh Chintalapati1, J S S Prakash1, Ashish K Singh1, Shuji Ohtani2, Iwane Suzuki3, Norio Murata4, Sisinthy Shivaji5.
Abstract
Antarctic psychrotolerant, Nostoc sp. (SO-36), when grown at 25 degrees C and then shifted to 10 degrees C, showed an increase in the tri-unsaturated fatty acid [C(18:3(9,12,15))] at the expense of mono- [C(18:1(9))] and di-unsaturated [C(18:2(9,12))] fatty acids. These results indicate that the activities of the enzymes DesA and DesB are up-regulated, when cultures were grown at 10 degrees C or shifted to 10 degrees C from 25 degrees C. However, RT-PCR studies indicated a constitutive expression of desA, desB, desC, and desC2 genes when cultures grown at 25 degrees C were shifted to 10 degrees C. This constitutive expression of des genes is in contrast to that observed in mesophilic cyanobacteria, in which desA and desB are transcriptionally up-regulated in response to lowering of growth temperature.Entities:
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Year: 2007 PMID: 17697671 DOI: 10.1016/j.bbrc.2007.07.150
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN: 0006-291X Impact factor: 3.575