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Literature DB >> 1769563

Time-dependent AluI action on human chromosomes.

A M Fernández-Peralta¹, I Tagarro, P Ludeña Reyes, C Sentís Castaño, J Fernández Piqueras, J J González-Aguilera.

Abstract

We have analyzed the pattern of AluI digestion over time on human chromosomes in order to monitor the evolution of the in situ enzyme action. Short treatments followed by Giemsa staining produce a G-like banding effect, whereas longer treatments produce a C-like banding pattern. However, when Propidium iodide staining is used, it reveals a uniform bright fluorescence after short AluI digestions and C bands when longer treatments are developed. We propose that C banding is the result of a uniform DNA removal in non centromeric regions taking place after a critical time point, the initial G like banding being produced by changes in the DNA-proteins interactions.

Entities: Chemical Species

Mesh：

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Year: 1991 PMID： 1769563 DOI： 10.1007/bf00127246

Source DB: PubMed Journal: Genetica ISSN： 0016-6707 Impact factor: 1.082

25 in total

10. Differential decondensation of mitotic chromosomes during hypotonic treatment of living cells as a possible cause of G-banding: an ultrastructural study.

Authors: O V Zatsepina; V Y Polyakov; Y S Chentsov
Journal: Chromosoma Date: 1989-08 Impact factor: 4.316

1 in total

1. Restriction endonuclease/nick translation procedure on fixed chromosomes of the Atlantic salmon fish cell line.

Authors: M Abuín; P Martínez; L Sánchez
Journal: Chromosome Res Date: 1995-09 Impact factor: 5.239

1 in total

Time-dependent AluI action on human chromosomes.

1. Some factors affecting the action of restriction endonucleases on human metaphase chromosomes.

2. Morphological and biochemical effects of endonucleases on isolated mammalian chromosomes in vitro.

3. The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes.

4. Endonuclease banding of isolated mammalian metaphase chromosomes.

5. The heteromorphic marker on chromosome 18 using restriction endonuclease AluI.

6. Mechanisms of chromosome banding. II. Evidence that histones are not involved.

7. Chromosome localization of highly repetitive human DNA's and amplified ribosomal DNA with restriction enzymes.

Review 8. The nature and mechanisms of chromosome banding.

9. The effect of restriction enzyme digestion of human metaphase chromosomes on C-band variants of chromosomes 1 and 9.

10. Differential decondensation of mitotic chromosomes during hypotonic treatment of living cells as a possible cause of G-banding: an ultrastructural study.

1. Restriction endonuclease/nick translation procedure on fixed chromosomes of the Atlantic salmon fish cell line.