A Bayram1, S Erkilic, A Ozkur, M Bayram, I Sari. 1. Department of Microbiology and Clinical Microbiology, School of Medicine, University of Gaziantep, Turkey. aysenbayram@hotmail.com
Abstract
AIMS: This study was conducted to evaluate the relationship between total intrahepatic hepatitis B virus (HBV) DNA levels and liver histology in terms of the degree of histological activity index (HAI) that yields necroinflammation (HAI-NI) and fibrosis (HAI-F) of the liver. METHODS: Prospectively, Tru-cut needle biopsy samples were obtained from the livers of 42 patients with chronic hepatitis B. Levels of serum and liver HBV DNA were determined by quantitative real-time PCR. Demographic data of patients, together with hepatitis B serology, alanine aminotransferase levels, and HAI-NI and HAI-F scores, were recorded. RESULTS: Twenty of the patients were hepatitis B e antigen (HBeAg) positive, while 22 patients were positive for antibody to HBeAg (anti-HBe). Serum and liver total HBV DNA levels were found to correlate directly with each other in the two groups (r = 0.669, p = 0.001; and r = 0.880, p<0.001; respectively) and the correlation was more marked in anti-HBe-positive patients. Although serum HBV DNA levels correlated positively with HAI-NI and HAI-F scores in HBeAg-positive and HBeAg-negative patients, total liver HBV DNA levels correlated with HAI-NI and HAI-F scores in anti-HBe-positive patients only. CONCLUSIONS: Quantitative measurement of intrahepatic HBV DNA is a valuable marker of the histological status of the liver in anti-HBe-positive patients with chronic hepatitis B, and it may give an insight into the prognosis and the ideal time for the cessation of antiviral treatment.
AIMS: This study was conducted to evaluate the relationship between total intrahepatic hepatitis B virus (HBV) DNA levels and liver histology in terms of the degree of histological activity index (HAI) that yields necroinflammation (HAI-NI) and fibrosis (HAI-F) of the liver. METHODS: Prospectively, Tru-cut needle biopsy samples were obtained from the livers of 42 patients with chronic hepatitis B. Levels of serum and liver HBV DNA were determined by quantitative real-time PCR. Demographic data of patients, together with hepatitis B serology, alanine aminotransferase levels, and HAI-NI and HAI-F scores, were recorded. RESULTS: Twenty of the patients were hepatitis B e antigen (HBeAg) positive, while 22 patients were positive for antibody to HBeAg (anti-HBe). Serum and liver total HBV DNA levels were found to correlate directly with each other in the two groups (r = 0.669, p = 0.001; and r = 0.880, p<0.001; respectively) and the correlation was more marked in anti-HBe-positive patients. Although serum HBV DNA levels correlated positively with HAI-NI and HAI-F scores in HBeAg-positive and HBeAg-negative patients, total liver HBV DNA levels correlated with HAI-NI and HAI-F scores in anti-HBe-positive patients only. CONCLUSIONS: Quantitative measurement of intrahepatic HBV DNA is a valuable marker of the histological status of the liver in anti-HBe-positive patients with chronic hepatitis B, and it may give an insight into the prognosis and the ideal time for the cessation of antiviral treatment.
Authors: Romina Salpini; Lorenzo Piermatteo; Upkar Gill; Arianna Battisti; Francesca Stazi; Tania Guenci; Sara Giannella; Valentina Serafini; Patrick T F Kennedy; Carlo Federico Perno; Valentina Svicher; Marco Ciotti Journal: Med Microbiol Immunol Date: 2017-04-11 Impact factor: 3.402
Authors: S Pradeep Kumar; Subhash Medhi; Mohammad Asim; Bhudev C Das; Ranjana Gondal; Premashis Kar Journal: Indian J Med Res Date: 2011-01 Impact factor: 2.375
Authors: Daniel Stone; Kelly R Long; Michelle A Loprieno; Harshana S De Silva Feelixge; Elizabeth J Kenkel; R Matt Liley; Stephen Rapp; Pavitra Roychoudhury; Thuy Nguyen; Laurence Stensland; Rossana Colón-Thillet; Lindsay M Klouser; Nicholas D Weber; Connie Le; Jessica Wagoner; Erin A Goecker; Alvason Zhenhua Li; Karsten Eichholz; Lawrence Corey; D Lorne Tyrrell; Alexander L Greninger; Meei-Li Huang; Stephen J Polyak; Martine Aubert; John E Sagartz; Keith R Jerome Journal: Mol Ther Methods Clin Dev Date: 2020-11-26 Impact factor: 6.698