Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Contribution of the FtsQ transmembrane segment to localization to the cell division site.

Literature DB >> 17693520

Contribution of the FtsQ transmembrane segment to localization to the cell division site.

Dirk-Jan Scheffers¹, Carine Robichon, Gert Jan Haan, Tanneke den Blaauwen, Gregory Koningstein, Edwin van Bloois, Jon Beckwith, Joen Luirink.

Abstract

The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.

Entities: Chemical Gene Mutation Species

Mesh：

Substances：

Year: 2007 PMID： 17693520 PMCID： PMC2168451 DOI： 10.1128/JB.00723-07

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

40 in total

1. A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery.

Authors: H Tian; D Boyd; J Beckwith
Journal: Proc Natl Acad Sci U S A Date: 2000-04-25 Impact factor: 11.205

2. Analysis of ftsQ mutant alleles in Escherichia coli: complementation, septal localization, and recruitment of downstream cell division proteins.

Authors: Joseph C Chen; Michael Minev; Jon Beckwith
Journal: J Bacteriol Date: 2002-02 Impact factor: 3.490

3. Escherichia coli minicell membranes are enriched in cardiolipin.

Authors: C M Koppelman; T Den Blaauwen; M C Duursma; R M Heeren; N Nanninga
Journal: J Bacteriol Date: 2001-10 Impact factor: 3.490

4. Versatility of inner membrane protein biogenesis in Escherichia coli.

Authors: Linda Fröderberg; Edith Houben; James C Samuelson; Minyong Chen; Sei-Kyoung Park; Gregory J Phillips; Ross Dalbey; Joen Luirink; Jan-Willem L De Gier
Journal: Mol Microbiol Date: 2003-02 Impact factor: 3.501

5. Evidence that subcellular localization of a bacterial membrane protein is achieved by diffusion and capture.

Authors: David Z Rudner; Qi Pan; Richard M Losick
Journal: Proc Natl Acad Sci U S A Date: 2002-06-11 Impact factor: 11.205

6. GALLEX, a measurement of heterologous association of transmembrane helices in a biological membrane.

Authors: Dirk Schneider; Donald M Engelman
Journal: J Biol Chem Date: 2002-11-21 Impact factor: 5.157

7. Penicillin-binding protein PBP2 of Escherichia coli localizes preferentially in the lateral wall and at mid-cell in comparison with the old cell pole.

Authors: Tanneke Den Blaauwen; Mirjam E G Aarsman; Norbert O E Vischer; Nanne Nanninga
Journal: Mol Microbiol Date: 2003-01 Impact factor: 3.501

8. Bacterial birth scar proteins mark future flagellum assembly site.

Authors: Edgar Huitema; Sean Pritchard; David Matteson; Sunish Kumar Radhakrishnan; Patrick H Viollier
Journal: Cell Date: 2006-03-10 Impact factor: 41.582

9. Sec-dependent membrane protein insertion: sequential interaction of nascent FtsQ with SecY and YidC.

Authors: M L Urbanus; P A Scotti; L Froderberg; A Saaf; J W de Gier; J Brunner; J C Samuelson; R E Dalbey; B Oudega; J Luirink
Journal: EMBO Rep Date: 2001-06 Impact factor: 8.807

10. YgbQ, a cell division protein in Escherichia coli and Vibrio cholerae, localizes in codependent fashion with FtsL to the division site.

Authors: Nienke Buddelmeijer; Nicholas Judson; Dana Boyd; John J Mekalanos; Jonathan Beckwith
Journal: Proc Natl Acad Sci U S A Date: 2002-04-23 Impact factor: 11.205

12 in total

1. Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis.

Authors: Simon J Jakubowski; Jennifer E Kerr; Isaac Garza; Vidhya Krishnamoorthy; Richard Bayliss; Gabriel Waksman; Peter J Christie
Journal: Mol Microbiol Date: 2008-12-01 Impact factor: 3.501

2. Divisome under construction: distinct domains of the small membrane protein FtsB are necessary for interaction with multiple cell division proteins.

Authors: Mark D Gonzalez; Jon Beckwith
Journal: J Bacteriol Date: 2009-02-20 Impact factor: 3.490

3. FtsW is a dispensable cell division protein required for Z-ring stabilization during sporulation septation in Streptomyces coelicolor.

Authors: Bhavesh V Mistry; Ricardo Del Sol; Chris Wright; Kim Findlay; Paul Dyson
Journal: J Bacteriol Date: 2008-06-13 Impact factor: 3.490

4. Structure of CrgA, a cell division structural and regulatory protein from Mycobacterium tuberculosis, in lipid bilayers.

Authors: Nabanita Das; Jian Dai; Ivan Hung; Malini R Rajagopalan; Malini R Rajagopalan; Huan-Xiang Zhou; Timothy A Cross
Journal: Proc Natl Acad Sci U S A Date: 2014-12-29 Impact factor: 11.205

5. Multiple interaction domains in FtsL, a protein component of the widely conserved bacterial FtsLBQ cell division complex.

Authors: Mark D Gonzalez; Esra A Akbay; Dana Boyd; Jon Beckwith
Journal: J Bacteriol Date: 2010-04-02 Impact factor: 3.490

6. The Soluble Periplasmic Domains of Escherichia coli Cell Division Proteins FtsQ/FtsB/FtsL Form a Trimeric Complex with Submicromolar Affinity.

Authors: Marjolein Glas; H Bart van den Berg van Saparoea; Stephen H McLaughlin; Winfried Roseboom; Fan Liu; Gregory M Koningstein; Alexander Fish; Tanneke den Blaauwen; Albert J R Heck; Luitzen de Jong; Wilbert Bitter; Iwan J P de Esch; Joen Luirink
Journal: J Biol Chem Date: 2015-07-09 Impact factor: 5.157