| Literature DB >> 17692983 |
Rebekka Biedendieck1, Martin Gamer, Lothar Jaensch, Steffen Meyer, Manfred Rohde, Wolf-Dieter Deckwer, Dieter Jahn.
Abstract
A sucrose-inducible promoter system (P(sacB)) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l(-1)) and the heterologous green fluorescent protein GFP (7.9 mg g(CDW)(-1)). Mutational analysis of B. megaterium P(sacB) allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P(xylA)) for recombinant gene expression in B. megaterium.Entities:
Mesh：
Substances：
Year: 2007 PMID： 17692983 DOI： 10.1016/j.jbiotec.2007.07.494
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307